| CPC G01N 35/1002 (2013.01) [G01N 35/0099 (2013.01); G01N 35/1009 (2013.01); H01J 49/164 (2013.01)] | 6 Claims |
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1. A sample pretreatment device, comprising:
a plurality of containers including a plurality of first well plates, each first plate of the plurality of first plates includes wells formed in a matrix form with N rows and M columns (where both N and M are integers equal to or greater than two), and a second well plate in which N elongated wells whose length corresponds to a length of a space in which the M wells in one row of the first well plate are formed, or in which M elongated wells whose length corresponds to a length of a space in which the N wells in one column of the first well plate are formed, a plurality of reagent solutions for a pretreatment are respectively contained in L elongated wells of the second well plate (where L is an integer equal to or greater than two and equal to or less than N or M;
a container storage section capable of holding the plurality of containers;
a dispensing section including a working platform on which the containers are configured to be placed, a pipette unit including M-channel tips corresponding to the M wells in one row of the first well plate or N-channel tips corresponding to the N wells in one column of the first well plate, and a pump unit configured to suction and eject a liquid from and into each container of the plurality of containers placed on the working platform through the pipette unit;
a transfer section configured to transfer the containers between the container storage section and the working platform; and
a controller programmed to control an operation of the dispensing section and the transfer section so as to perform following operations:
a transferring operation in which the second well plate and the plurality of first well plates are individually transferred from the container storage section to the working platform;
a reagent-solution-dispensing operation in which a dispensing operation is performed for each of the plurality of reagent solutions contained in the elongated wells of the second well plate, where the dispensing operation includes inserting the M-channel tips or the N-channel tips into a first elongated well of the plurality of elongated wells and suctioning a first reagent solution of the plurality of reagent solutions contained in the first elongated well using the M-channel tips or the N-channel tips and dispensing the suctioned first reagent solution into wells of one first well plate of the first well plates using the M-channel tips or the N-channel tips, and inserting the M-channel tips or the N-channel tips into a second elongated well of the plurality of elongated wells and suctioning a second reagent solution of the plurality of reagent solutions contained in the second elongated well using the M-channel tips or the N-channel tips and dispensing the suctioned second reagent solution into wells of another first well plate of the plurality of first well plates using the M-channel tips or the N-channel tips;
a sample-injecting operation, performed on the working platform, in which a plurality of different samples are individually injected into the wells of the one first well plate including the first reagent solution to provide mixed solutions, respectively, via the pipette unit using the M-channel tips or the N-channel tips or using a different multichannel tip; and
a mixed-solution-injecting operation, performed on the working platform after the sample-injecting operation, in which the mixed solutions are respectively suctioned from each well of the one first well plate and injected into the wells of the other first well plate including the second reagent solution via the pipette unit using the M-channel tips or the N-channel tips or using the different multichannel tip;
a sample-preparing section configured to prepare samples for matrix assisted laser desorption/ionization (MALDI) from the plurality of pretreated solutions on the working platform, by dropping each of the solutions onto a sample plate for MALDI and mixing each of the solutions with a matrix on the sample plate.
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