CPC G01N 33/581 (2013.01) [C12Q 1/6837 (2013.01); G01N 33/543 (2013.01); G01N 33/54346 (2013.01); G01N 33/54366 (2013.01); G01N 33/54386 (2013.01); G01N 2333/938 (2013.01)] | 24 Claims |
1. A system for determining a concentration of a target analyte in a sample, comprising:
an optical detection system configured to identify optical signatures at individual assay locations on a substrate; and
a computer that executes imaging processing software configured to process information from the optical detection system and to:
perform a binary readout method to determine a percentage of a total number of the assay locations at which an optical detectable signal is observed by the optical detection system; and
correlate the percentage of assay locations at which the detectable signal is present to a bulk concentration of the target analyte in the sample.
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