US 11,874,279 B2
Methods and arrays for target analyte detection and determination of target analyte concentration in solution
David R. Walt, Boston, MA (US); and David M. Rissin, Lexington, MA (US)
Assigned to Trustees of Tufts College, Medford, MA (US)
Filed by Trustees of Tufts College, Medford, MA (US)
Filed on Oct. 8, 2021, as Appl. No. 17/496,926.
Application 17/496,926 is a continuation of application No. 16/286,811, filed on Feb. 27, 2019, granted, now 11,237,171.
Application 16/286,811 is a continuation of application No. 15/205,497, filed on Jul. 8, 2016, granted, now 10,261,089.
Application 15/205,497 is a continuation of application No. 14/638,245, filed on Mar. 4, 2015, granted, now 9,395,359.
Application 14/638,245 is a continuation of application No. 13/870,596, filed on Apr. 25, 2013, abandoned.
Application 13/870,596 is a continuation of application No. 11/707,385, filed on Feb. 16, 2007, granted, now 8,460,879.
Application 13/870,596 is a continuation of application No. 11/707,383, filed on Feb. 16, 2007, granted, now 8,460,878.
Application 13/870,956 is a continuation of application No. 11/707,384, filed on Feb. 16, 2007, granted, now 8,492,098.
Claims priority of provisional application 60/792,736, filed on Apr. 17, 2006.
Claims priority of provisional application 60/775,692, filed on Feb. 21, 2006.
Prior Publication US 2022/0099678 A1, Mar. 31, 2022
Int. Cl. G01N 33/58 (2006.01); C12Q 1/6837 (2018.01); G01N 33/543 (2006.01)
CPC G01N 33/581 (2013.01) [C12Q 1/6837 (2013.01); G01N 33/543 (2013.01); G01N 33/54346 (2013.01); G01N 33/54366 (2013.01); G01N 33/54386 (2013.01); G01N 2333/938 (2013.01)] 24 Claims
OG exemplary drawing
 
1. A system for determining a concentration of a target analyte in a sample, comprising:
an optical detection system configured to identify optical signatures at individual assay locations on a substrate; and
a computer that executes imaging processing software configured to process information from the optical detection system and to:
perform a binary readout method to determine a percentage of a total number of the assay locations at which an optical detectable signal is observed by the optical detection system; and
correlate the percentage of assay locations at which the detectable signal is present to a bulk concentration of the target analyte in the sample.