| CPC G01N 21/51 (2013.01) [G01N 1/2035 (2013.01); G01N 21/534 (2013.01); G02B 5/04 (2013.01); G01N 2021/1765 (2013.01); G01N 2021/4769 (2013.01)] | 22 Claims |
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1. A device comprising:
a flow cell, and a fluid containing samples flowing in the flow cell;
an irradiation optical system that irradiates the samples flowing in the flow cell with planar light at an incidence surface, the planer light being irradiated orthogonal to a Y flow direction in which the samples flow;
an imaging optical system having an observation axis inclined with respect to an irradiation surface for the planar light, the imaging optical system forms an image of observation light generated in the sample due to the irradiation with the planar light, the incidence surface of the planar light and an irradiation surface of the observation light, in the flow cell, are the same surface, a side surface of a tube constituting the flow cell is the incidence surface of the planar light and the irradiation surface of the observation light, and an inclination angle of the observation axis with respect to the irradiation surface for the planar light is 30° to 65°;
a two-dimensional image sensor that captures a plurality of XZ light images of a XZ plane orthogonal to the Y flow direction including at least a cross section of the fluid among XZ light images according to the observation light formed by the image optical system, and outputs the plurality of XZ light images of the XZ plane orthogonal to the Y flow direction as image data, the plurality of XZ light images of the XZ plane orthogonal to the Y flow direction capturing the samples flowing in the Y flow direction and capturing the samples passing the planer light by observing the planer light with the inclination angle of the observation axis, wherein the irradiation optical system, the imaging optical system, and the two-dimensional image sensor are located on a same side from the flow cell; and
a computer that analyzes a light intensity profile of the sample in the Y flow direction of the fluid on the basis of the image data,
wherein the imaging optical system includes an objective lens, and a first prism disposed between the flow cell and the objective lens, and
the first prism has a refractive index greater than that of a disposition space of the imaging optical system, and the first prism increases a second inclination angle with respect to the irradiation surface for the planar light of the observation light emitted from the sample as compared with a case in which the first prism is not disposed,
the first prism is a non-axisymmetric prism that bends rays on one axis of the observation light with a predetermined declination according to an incident position and does not bend rays on an axis orthogonal to the one axis, and
the imaging optical system includes an image formation lens, the image formation lens forms an image of the observation light from the object lens on the two-dimensional image sensor.
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