US 11,874,216 B2
Automatic reactive oxygen species content detection system suitable for cell microenvironment
Yongdong Dai, Zhejiang (CN); Lulu Liu, Zhejiang (CN); Songying Zhang, Zhejiang (CN); Xiaomei Tong, Zhejiang (CN); Xiaona Lin, Zhejiang (CN); Xiang Lin, Zhejiang (CN); and Yonghang Shen, Zhejiang (CN)
Assigned to ZHEJIANG UNIVERSITY, Zhejiang (CN)
Appl. No. 17/292,540
Filed by ZHEJIANG UNIVERSITY, Zhejiang (CN)
PCT Filed Sep. 25, 2020, PCT No. PCT/CN2020/117629
§ 371(c)(1), (2) Date May 10, 2021,
PCT Pub. No. WO2021/208366, PCT Pub. Date Oct. 21, 2021.
Claims priority of application No. 202010289512.X (CN), filed on Apr. 14, 2020.
Prior Publication US 2022/0236167 A1, Jul. 28, 2022
Int. Cl. G01N 21/11 (2006.01); G01N 21/64 (2006.01); G01N 21/03 (2006.01)
CPC G01N 21/11 (2013.01) [G01N 21/6428 (2013.01); G01N 2021/0325 (2013.01); G01N 2021/0346 (2013.01); G01N 2021/115 (2013.01); G01N 2021/6417 (2013.01); G01N 2021/6439 (2013.01); G01N 2021/6482 (2013.01)] 10 Claims
OG exemplary drawing
 
1. An automatic reactive oxygen species content detection system suitable for a cell microenvironment, comprising:
a sample transmission reaction system and a detection system which are communicated in sequence through a light avoiding pipeline, the sample transmission reaction system having a sample injector and a DCFH supply bin which are in communication with a reaction bin through light avoiding pipelines after being connected in parallel, a first sample injection valve is positioned inline of the light avoiding pipes between the sample injector and the reaction bin and a second sample injection valve is positioned inline of the light avoiding pipes between the DCFH supply bin and the reaction bin;
a washing system which is communicated with the sample transmission reaction system through a water pipeline, the washing system having a water supply bin and water pipelines connecting the water supply bin with each of the sample injector and the DCFH supply bin, a first washing pump is positioned inline of the water pipelines between the sample injector and water supply bin and a second washing pump is positioned inline of the water pipelines between the sample injector and the DCFH supply bin; and
a purge system which is communicated with the sample transmission reaction system through a gas pipeline the purge system having a gas source and gas pipelines connecting the gas source each of the sample injector and the DCFH supply bin, a first gas pressure valve is positioned inline of the gas pipelines between the sample injector and the gas source, and a second gas pressure valve positioned inline of the gas pipelines between the DCFH supply bin and the gas source;
the detection system having:
a light transmitting microfluidic pipe and a waste liquid collector in communication with reaction bin through a light avoiding pipeline system; and
a fluorescence detector provided with a light source is configured to detect a sample in the light transmitting microfluidic pipe; the fluorescence detector generating a light signal converted to an electric signal by a photoelectric converter that is transmitted to a data processing terminal.