CPC G01N 21/11 (2013.01) [G01N 21/6428 (2013.01); G01N 2021/0325 (2013.01); G01N 2021/0346 (2013.01); G01N 2021/115 (2013.01); G01N 2021/6417 (2013.01); G01N 2021/6439 (2013.01); G01N 2021/6482 (2013.01)] | 10 Claims |
1. An automatic reactive oxygen species content detection system suitable for a cell microenvironment, comprising:
a sample transmission reaction system and a detection system which are communicated in sequence through a light avoiding pipeline, the sample transmission reaction system having a sample injector and a DCFH supply bin which are in communication with a reaction bin through light avoiding pipelines after being connected in parallel, a first sample injection valve is positioned inline of the light avoiding pipes between the sample injector and the reaction bin and a second sample injection valve is positioned inline of the light avoiding pipes between the DCFH supply bin and the reaction bin;
a washing system which is communicated with the sample transmission reaction system through a water pipeline, the washing system having a water supply bin and water pipelines connecting the water supply bin with each of the sample injector and the DCFH supply bin, a first washing pump is positioned inline of the water pipelines between the sample injector and water supply bin and a second washing pump is positioned inline of the water pipelines between the sample injector and the DCFH supply bin; and
a purge system which is communicated with the sample transmission reaction system through a gas pipeline the purge system having a gas source and gas pipelines connecting the gas source each of the sample injector and the DCFH supply bin, a first gas pressure valve is positioned inline of the gas pipelines between the sample injector and the gas source, and a second gas pressure valve positioned inline of the gas pipelines between the DCFH supply bin and the gas source;
the detection system having:
a light transmitting microfluidic pipe and a waste liquid collector in communication with reaction bin through a light avoiding pipeline system; and
a fluorescence detector provided with a light source is configured to detect a sample in the light transmitting microfluidic pipe; the fluorescence detector generating a light signal converted to an electric signal by a photoelectric converter that is transmitted to a data processing terminal.
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