US 11,873,524 B2
Methods and systems for the rapid detection of Salmonella using infectious agents
Stephen Erickson, White Bear Township, MN (US); Jose S. Gil, Winnetka, CA (US); Minh Mindy Bao Nguyen, Shoreview, MN (US); Dwight Lyman Anderson, Minneapolis, MN (US); and Jessica Stach, St. Paul, MN (US)
Assigned to Laboratory Corporation of America Holdings, Burlington, NC (US)
Filed by Laboratory Corporation of America Holdings, Burlington, NC (US)
Filed on Jan. 14, 2019, as Appl. No. 16/247,486.
Claims priority of provisional application 62/661,739, filed on Apr. 24, 2018.
Claims priority of provisional application 62/628,616, filed on Feb. 9, 2018.
Claims priority of provisional application 62/616,956, filed on Jan. 12, 2018.
Prior Publication US 2019/0218589 A1, Jul. 18, 2019
Int. Cl. C12Q 1/10 (2006.01); C12N 15/86 (2006.01); C12N 15/65 (2006.01); C12Q 1/70 (2006.01); G01N 33/50 (2006.01); C12Q 1/66 (2006.01); A61K 35/76 (2015.01); C12N 15/74 (2006.01); C12N 7/00 (2006.01); C12Q 1/6897 (2018.01); C12Q 1/02 (2006.01)
CPC C12Q 1/10 (2013.01) [A61K 35/76 (2013.01); C12N 7/00 (2013.01); C12N 15/65 (2013.01); C12N 15/74 (2013.01); C12N 15/86 (2013.01); C12Q 1/025 (2013.01); C12Q 1/66 (2013.01); C12Q 1/6897 (2013.01); C12Q 1/70 (2013.01); G01N 33/50 (2013.01); C12N 2795/00021 (2013.01); C12N 2795/00031 (2013.01); C12N 2795/00051 (2013.01); C12N 2795/10131 (2013.01); C12N 2795/10143 (2013.01); C12Q 2304/60 (2013.01); G01N 2800/52 (2013.01)] 6 Claims
OG exemplary drawing
 
1. A method for detecting Salmonella spp. in a sample comprising:
incubating a sample comprising powdered infant formula, for 30-120 minutes with a recombinant bacteriophage derived from a Salmonella-specific bacteriophage comprising, a genetic construct inserted into a late gene region of the bacteriophage genome, the genetic construct comprising:
(i) an indicator gene; and
(ii) a promoter controlling transcription of the indicator gene, wherein the promoter is a bacteriophage late promoter;
and wherein the indicator gene is not contiguous with a gene encoding a structural bacteriophage protein and does not yield a fusion protein, and wherein expression of the indicator gene results in an indicator protein product; and
detecting the indicator protein product, wherein positive detection of the indicator protein product indicates that Salmonella spp. is present in the sample.