US 11,873,522 B2
Solubilization of MSW with blend enzymes
Hanne Risbjerg Soerensen, Holte (DK); Lisa Rosgaard, Valby (DK); Henrik B. Nielsen, Bagsværd (DK); Lone Baekgaard, Bagsværd (DK); and Joanna Wawrzynczyk, Bagsværd (DK)
Assigned to RENESCIENCE A/S, Fredericia (DK)
Filed by Renescience A/S, Fredericia (DK)
Filed on Apr. 26, 2021, as Appl. No. 17/240,453.
Application 17/240,453 is a division of application No. 15/506,881, granted, now 11,124,808, previously published as PCT/EP2015/069685, filed on Aug. 27, 2015.
Claims priority of application No. 14182698 (EP), filed on Aug. 28, 2014.
Prior Publication US 2021/0348199 A1, Nov. 11, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 7/10 (2006.01); C11D 3/386 (2006.01); C12N 9/20 (2006.01); C12N 9/26 (2006.01); C12N 9/42 (2006.01); C12N 9/24 (2006.01); C12N 9/48 (2006.01); C12N 9/54 (2006.01); C12N 9/88 (2006.01); C12P 5/02 (2006.01); C12P 7/08 (2006.01)
CPC C12P 7/10 (2013.01) [C11D 3/38618 (2013.01); C11D 3/38627 (2013.01); C11D 3/38645 (2013.01); C12N 9/20 (2013.01); C12N 9/244 (2013.01); C12N 9/2411 (2013.01); C12N 9/2434 (2013.01); C12N 9/2448 (2013.01); C12N 9/2488 (2013.01); C12N 9/485 (2013.01); C12N 9/54 (2013.01); C12N 9/88 (2013.01); C12P 5/023 (2013.01); C12P 7/08 (2013.01); C12Y 301/01 (2013.01); C12Y 302/01 (2013.01); C12Y 302/01006 (2013.01); C12Y 302/01073 (2013.01); C12Y 304/11001 (2013.01); C12Y 304/21004 (2013.01); C12Y 304/23023 (2013.01); C12Y 304/23028 (2013.01)] 21 Claims
 
1. A process for producing a fermentation product, comprising:
(a) treating Municipal Solid Waste (MSW) with an enzyme composition comprising a cellulolytic background composition present at a ratio between 40-99% w/w of the total enzyme protein, and further comprising:
ii) a protease present at a ratio between 5-15% w/w of the total enzyme protein;
iii) a lipase present at a ratio between 2.5-7.5% w/w of the total enzyme protein; and/or
iv) a beta-glucanase present at a ratio between 10-20% w/w of the total enzyme protein; and wherein the enzyme composition further comprises one or more enzymes selected from the group consisting of a pectate lyase, a mannanase, and an amylase;
(b) fermenting the solubilized and/or hydrolysed MSW with one or more fermenting microorganisms to produce a fermentation product; and
(c) recovering the fermentation product from the fermentation.