US 11,873,520 B2
Production of malonyl-COA derived products via anaerobic pathways
William Ryan Sillers, Lebanon, NH (US); Shital A. Tripathi, Berkeley, CA (US); Arthur J. Shaw, IV, Grantham, NH (US); Aaron Argyros, White River Junction, VT (US); and David A. Hogsett, Grantham, NH (US)
Assigned to Lallemand Hungary Liquidity Management LLC, Budapest (HU)
Filed by Lallemand Hungary Liquidity Management LLC, Budapest (HU)
Filed on Sep. 30, 2021, as Appl. No. 17/491,268.
Application 17/491,268 is a continuation of application No. 16/166,274, filed on Oct. 22, 2018, granted, now 11,162,125.
Application 16/166,274 is a continuation of application No. 13/814,616, granted, now 10,138,504, issued on Nov. 27, 2018, previously published as PCT/US2011/046869, filed on Aug. 5, 2011.
Claims priority of provisional application 61/371,582, filed on Aug. 6, 2010.
Prior Publication US 2022/0267816 A1, Aug. 25, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 17/18 (2006.01); C12N 1/22 (2006.01); C12N 9/10 (2006.01); C12N 9/88 (2006.01); C12P 7/6409 (2022.01)
CPC C12P 17/182 (2013.01) [C12N 1/22 (2013.01); C12N 9/1018 (2013.01); C12N 9/88 (2013.01); C12P 7/6409 (2013.01); G01N 2333/91034 (2013.01)] 27 Claims
 
1. A recombinant yeast microorganism comprising one or more engineered metabolic pathways to convert a carbohydrate source to ethanol and a malonyl-CoA derived product, wherein the one or more engineered metabolic pathways comprises (a) the conversion of phosphoenolpyruvate to oxaloacetate by a phosphoenolpyruvate carboxykinase and (b) the conversion of oxaloacetate and acetyl-CoA to malonyl-CoA and pyruvate by a heterologous transcarboxylase Enzyme Commission Number 2.1.3.1; wherein the one or more engineered metabolic pathways further comprises heterologous pyruvate formate lyase.