US 11,873,496 B2
Methods of altering gene expression by perturbing transcription factor multimers that structure regulatory loops
Richard A. Young, Boston, MA (US); Abraham S. Weintraub, Somerville, MA (US); Charles H. Li, Waban, MA (US); and Alla A. Sigova, Newton, MA (US)
Assigned to Whitehead Institute for Biomedical Research, Cambridge, MA (US)
Appl. No. 16/476,868
Filed by Whitehead Institute for Biomedical Research, Cambridge, MA (US)
PCT Filed Jan. 9, 2018, PCT No. PCT/US2018/013003
§ 371(c)(1), (2) Date Jul. 9, 2019,
PCT Pub. No. WO2018/129544, PCT Pub. Date Jul. 12, 2018.
Claims priority of provisional application 62/596,093, filed on Dec. 7, 2017.
Claims priority of provisional application 62/444,341, filed on Jan. 9, 2017.
Prior Publication US 2019/0352648 A1, Nov. 21, 2019
Int. Cl. C07H 21/02 (2006.01); C12N 15/67 (2006.01); C07K 14/47 (2006.01); C12N 15/113 (2010.01); G01N 33/50 (2006.01); C12N 15/85 (2006.01)
CPC C12N 15/67 (2013.01) [C07K 14/4702 (2013.01); C12N 15/113 (2013.01); C12N 15/85 (2013.01); G01N 33/5023 (2013.01); C12N 2015/859 (2013.01); C12N 2310/51 (2013.01); C12N 2310/53 (2013.01); C12N 2830/001 (2013.01); C12N 2830/15 (2013.01)] 18 Claims
 
1. A method of decreasing expression of one or more genes in a cell, comprising contacting the cell with a composition for decreasing binding of Yin Yang 1 (YY1) to a YY1 binding site, wherein the composition comprises a site-specific DNA binding domain (DBD) that binds a promoter or an enhancer of the one or more genes and an effector domain having DNA methylation activity, or one or more nucleic acids encoding the site-specific DBD and/or effector domain, and wherein the YY1 binding site is in the promoter or the enhancer of the one or more genes, thereby decreasing expression of the one or more genes.