US 11,873,481 B2
Preserving spatial-proximal contiguity and molecular contiguity in nucleic acid templates
Siddarth Selvaraj, San Diego, CA (US); Anthony Schmitt, Poway, CA (US); and Bret Reid, San Diego, CA (US)
Assigned to Arima Genomics, Inc., Carlsbad, CA (US)
Appl. No. 16/764,787
Filed by ARIMA GENOMICS, INC., San Diego, CA (US)
PCT Filed Nov. 20, 2018, PCT No. PCT/US2018/062005
§ 371(c)(1), (2) Date May 15, 2020,
PCT Pub. No. WO2019/104034, PCT Pub. Date May 31, 2019.
Claims priority of provisional application 62/589,505, filed on Nov. 21, 2017.
Prior Publication US 2021/0363516 A1, Nov. 25, 2021
Int. Cl. C12N 15/10 (2006.01); C12Q 1/6869 (2018.01)
CPC C12N 15/1065 (2013.01) [C12Q 1/6869 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A method for preparing library nucleic acid templates, comprising:
reacting isolated nucleic acid with one or more first reagents that generate proximity ligated nucleic acid molecules; and
reacting the proximity ligated nucleic acid molecules with one or more second reagents that fragment and attach barcode oligonucleotides to the proximity ligated nucleic acid molecules thereby providing barcoded templates that are in virtual compartments, wherein the virtual compartments are not physically barred from intermixing with other virtual compartments, and wherein the barcode oligonucleotides attached to the barcoded templates in one of the virtual compartments are distinguishable from the barcode oligonucleotides attached to the barcoded templates in other virtual compartments and barcodes in the barcode oligonucleotides preserve molecular contiguity information for proximity ligated molecules.