	US 11,873,472 B2
	Three-dimensional culture device and methods for dynamic culture of cell aggregates
Teng Ma, Tallahassee, FL (US); Ang-Chen Tsai, Tallahassee, FL (US); and Xuegang Yuan, Tallahassee, FL (US)
Assigned to FLORIDA STATE UNIVERSITY RESEARCH FOUNDATION, INC., Tallahassee, FL (US)
Filed by FLORIDA STATE UNIVERSITY RESEARCH FOUNDATION, INC., Tallahassee, FL (US); and Gary K. Ostrander, Tallahassee, FL (US)
Filed on Sep. 21, 2020, as Appl. No. 17/026,617.
Application 17/026,617 is a continuation of application No. 15/809,706, filed on Nov. 10, 2017, abandoned.
Prior Publication US 2021/0009932 A1, Jan. 14, 2021
Int. Cl. C12M 3/06 (2006.01); C12N 5/00 (2006.01); C12M 1/12 (2006.01)

CPC C12M 23/16 (2013.01) [C12M 25/14 (2013.01); C12M 27/16 (2013.01); C12N 5/0062 (2013.01); C12N 2513/00 (2013.01); C12N 2527/00 (2013.01); C12N 2533/12 (2013.01); C12N 2533/14 (2013.01); C12N 2533/30 (2013.01)]

16 Claims

1. A method for growing cells into aggregates of cells comprising culturing a cell in a micro-channel of a 3-D tissue culture insert provided in a tissue culture vessel, wherein said tissue culture vessel comprises one or more 3-D tissue culture inserts comprising one or more micro-channels that provide for a confined space for cells that are cultured in said one or more micro-channels and suitable culture media for culturing said cell, wherein said one or more micro-channels have a width of about 1 mm to about 5 mm, a length of about 20 mm to about 60 mm, and a height of about 5 mm to about 15 mm.