US 11,873,471 B2
Culture dish combination for embryo thawing and embryo transfer
Jin-peng Rao, Hangzhou (CN); Shen Tian, Hangzhou (CN); Chun Feng, Hangzhou (CN); Feng Qiu, Hangzhou (CN); Xiao-yun Wang, Hangzhou (CN); Fan Jin, Hangzhou (CN); and Min Jin, Hangzhou (CN)
Assigned to ZHEJIANG UNIVERSITY, Hangzhou (CN)
Filed by Zhejiang University, Hangzhou (CN)
Filed on Feb. 3, 2023, as Appl. No. 18/105,370.
Claims priority of application No. 202221063907.9 (CN), filed on May 6, 2022.
Prior Publication US 2023/0357687 A1, Nov. 9, 2023
Int. Cl. C12M 1/22 (2006.01); C12M 1/00 (2006.01)
CPC C12M 23/10 (2013.01) [C12M 23/22 (2013.01); C12M 23/34 (2013.01); C12M 23/38 (2013.01)] 9 Claims
OG exemplary drawing
 
1. A culture dish combination for embryo thawing and embryo transfer, comprising:
a first culture dish (1), comprising a first dish cavity (10) for thawing and laser-assisted hatching of a frozen embryo, and an opening of the first dish cavity (10) being upward; and
a second culture dish (2), detachably connected to the first culture dish (1), wherein the second culture dish (2) comprises a second dish cavity (20) for balancing culture medium before the embryo transfer and a third dish cavity (22) surrounding the second dish cavity (20), and openings of the second dish cavity (20) and the third dish cavity (22) are upward;
wherein the first culture dish (1) further comprises a first dish bottom (14) and a first annular wall (13) surrounding the first dish bottom (14), and the first dish bottom (14) is surrounded by the first annular wall (13) to define the first dish cavity (10); and an outer surface of the first annular wall (13) is provided with a convex portion (12) which protrudes outward along a direction perpendicular to an upward direction;
wherein the second culture dish (2) further comprises:
a second dish bottom (26);
a second annular wall (24), disposed on the second dish bottom (26);
a third annular wall (23), surrounding the second annular wall (24); and
an annular stepped portion (25), fixed on an inner wall of the second annular wall (24) facing away from the third annular wall (23);
wherein the annular stepped portion (25) comprises a fourth annular wall and a fifth annular wall, and the fourth annular wall is directly connected between the inner wall of the second annular wall (24) and the fifth annular wall; the third annular wall (23), the second annular wall (24) and the fifth annular wall are disposed on the second dish bottom (26) and arranged sequentially inwards from an outer edge of the second dish bottom (26), and heights of the third annular wall (23), the second annular wall (24) and the fifth annular wall gradually decrease; the second dish bottom (26) is surrounded by the second annular wall (24) to define the second dish cavity (20), the second dish bottom (26) is surrounded by the third annular wall (23) and the second annular wall (24) to define the third dish cavity (22) surrounding the second dish cavity (20) for maintaining humidity of the second dish cavity and washing residual oil droplets before the embryo transfer by adding embryo culture medium, and the second dish bottom (26) is surrounded by the fifth annular wall to define a central dish cavity (21) in the second dish cavity (20) for embryo aggregation.