US 11,873,312 B2
Transcriptome in vivo analysis (TIVA) and transcriptome in situ analysis (TISA)
James Eberwine, Philadelphia, PA (US); Ivan J. Dmochowski, Philadelphia, PA (US); Sean B. Yeldell, Philadelphia, PA (US); Julianne C. Griepenburg, Philadelphia, PA (US); Teresa L. Rapp, Philadelphia, PA (US); Jennifer M. Singh, Havertown, PA (US); Jai-Yoon Sul, Cherry Hill, NJ (US); and Jaehee Lee, Wynnewood, PA (US)
Assigned to The Trustees of the University of Pennsylvania, Philadelphia, PA (US)
Filed by The Trustees of the University of Pennsylvania, Philadelphia, PA (US)
Filed on May 28, 2021, as Appl. No. 17/333,376.
Application 17/333,376 is a division of application No. 15/501,073, granted, now 11,021,502, previously published as PCT/US2015/043581, filed on Aug. 4, 2015.
Claims priority of provisional application 62/033,007, filed on Aug. 4, 2014.
Claims priority of provisional application 62/035,410, filed on Aug. 9, 2014.
Prior Publication US 2021/0292352 A1, Sep. 23, 2021
Int. Cl. C12Q 1/6823 (2018.01); C12Q 1/6825 (2018.01); C12Q 1/6818 (2018.01); C07F 15/00 (2006.01); C12N 15/113 (2010.01); C07H 23/00 (2006.01); C12N 13/00 (2006.01); B01J 35/00 (2006.01)
CPC C07F 15/0053 (2013.01) [B01J 35/004 (2013.01); C07H 23/00 (2013.01); C12N 13/00 (2013.01); C12N 15/113 (2013.01); C12Q 1/6818 (2013.01); C12Q 1/6823 (2013.01); C12Q 1/6825 (2013.01); B01J 2531/821 (2013.01); C12N 2310/11 (2013.01); C12N 2310/32 (2013.01); C12N 2310/3233 (2013.01); C12N 2310/351 (2013.01); C12N 2310/3517 (2013.01); C12N 2310/531 (2013.01)] 9 Claims
OG exemplary drawing
 
1. A caged nucleic acid molecule comprising: a first, second, and third oligonucleotide, wherein the first oligonucleotide is linked to the second oligonucleotide through a first photocleavable linker and the second oligonucleotide is linked to the third oligonucleotide through a second photocleavable linker, wherein the first oligonucleotide comprises one or more terminal deoxynucleotides, wherein the first oligonucleotide comprises 2′-fluoro uridine, and wherein the one or more terminal deoxynucleotides comprises one or more thymidines.