US 11,872,635 B2
Bipyramid-templated synthesis of monodisperse noble metal nanocrystals
Yossi Weizmann, Chicago, IL (US); Kyle Gibson, Alma, MI (US); Jung-Hoon Lee, Chicago, IL (US); and Zoya Cheglakov, Chicago, IL (US)
Assigned to THE UNIVERSITY OF CHICAGO, Chicago, IL (US)
Filed by The University of Chicago, Chicago, IL (US)
Filed on Mar. 18, 2021, as Appl. No. 17/205,140.
Application 17/205,140 is a continuation of application No. 15/580,061, granted, now 11,045,874, previously published as PCT/US2016/031522, filed on May 9, 2016.
Claims priority of provisional application 62/172,481, filed on Jun. 8, 2015.
Prior Publication US 2021/0276095 A1, Sep. 9, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6806 (2018.01); B22F 9/24 (2006.01); B82Y 40/00 (2011.01); C30B 7/00 (2006.01); C30B 7/14 (2006.01); C30B 29/52 (2006.01); C30B 29/34 (2006.01); C30B 29/02 (2006.01); B22F 1/054 (2022.01); C30B 29/66 (2006.01); B82Y 20/00 (2011.01); B82Y 30/00 (2011.01); B22F 1/052 (2022.01)
CPC B22F 9/24 (2013.01) [B22F 1/0553 (2022.01); B82Y 40/00 (2013.01); C30B 7/005 (2013.01); C30B 7/14 (2013.01); C30B 29/02 (2013.01); C30B 29/34 (2013.01); C30B 29/52 (2013.01); C30B 29/66 (2013.01); B22F 1/052 (2022.01); B82Y 20/00 (2013.01); B82Y 30/00 (2013.01)] 11 Claims
OG exemplary drawing
 
1. A method for isothermal amplification of nucleic acid molecules, comprising:
preparing a reaction mixture that comprises the nucleic acid molecules, primer nucleic acid strands, free nucleotides, a nucleic acid polymerase, and a plurality of penta-twinned bipyramid-shaped noble metal nanocrystals in a nanocrystal sample, wherein at least 75% of the nanocrystals in the nanocrystal sample are the penta-twinned bipyramid-shaped noble metal nanocrystals;
irradiating the reaction mixture under a first pulse length and frequency, with radiation having wavelengths in an infrared or a visible region of the electromagnetic spectrum at which said nanocrystals absorb said radiation, thereby heating said reaction mixture to a first temperature for a first time period,
wherein said penta-twinned bipyramid-shaped noble metal nanocrystals are characterized by:
a plasmonic noble metal core and a PEGylated silica coating;
a size polydispersity of less than 10%;
and
an ability to absorb light having wavelengths in the range of 400-1200 nm.