CPC B22F 9/24 (2013.01) [B22F 1/0553 (2022.01); B82Y 40/00 (2013.01); C30B 7/005 (2013.01); C30B 7/14 (2013.01); C30B 29/02 (2013.01); C30B 29/34 (2013.01); C30B 29/52 (2013.01); C30B 29/66 (2013.01); B22F 1/052 (2022.01); B82Y 20/00 (2013.01); B82Y 30/00 (2013.01)] | 11 Claims |
1. A method for isothermal amplification of nucleic acid molecules, comprising:
preparing a reaction mixture that comprises the nucleic acid molecules, primer nucleic acid strands, free nucleotides, a nucleic acid polymerase, and a plurality of penta-twinned bipyramid-shaped noble metal nanocrystals in a nanocrystal sample, wherein at least 75% of the nanocrystals in the nanocrystal sample are the penta-twinned bipyramid-shaped noble metal nanocrystals;
irradiating the reaction mixture under a first pulse length and frequency, with radiation having wavelengths in an infrared or a visible region of the electromagnetic spectrum at which said nanocrystals absorb said radiation, thereby heating said reaction mixture to a first temperature for a first time period,
wherein said penta-twinned bipyramid-shaped noble metal nanocrystals are characterized by:
a plasmonic noble metal core and a PEGylated silica coating;
a size polydispersity of less than 10%;
and
an ability to absorb light having wavelengths in the range of 400-1200 nm.
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