US 11,867,894 B2
Method for SIM microscopy
Ingo Kleppe, Jena (DE); Yauheni Novikau, Apolda (DE); and Ralf Netz, Jena (DE)
Filed by Carl Zeiss Microscopy GmbH, Jena (DE)
Filed on Sep. 9, 2021, as Appl. No. 17/470,456.
Claims priority of application No. 10 2020 123 669.7 (DE), filed on Sep. 10, 2020.
Prior Publication US 2022/0075175 A1, Mar. 10, 2022
Int. Cl. G02B 21/36 (2006.01); G01N 21/64 (2006.01); G02B 21/06 (2006.01)
CPC G02B 21/367 (2013.01) [G01N 21/6458 (2013.01); G02B 21/06 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A method for performing Structured Illumination Microscopy (SIM) on a sample, comprising:
generating raw images of the sample, for each raw image by illuminating the sample using an identical SIM illumination pattern, with an individual shift for each raw image, wherein the SIM illumination pattern is characterized by p orders of diffraction, and
generating an image of the sample by evaluating n of the raw images in an image reconstruction,
wherein the image reconstruction comprises suppressing t highest orders of diffraction of the p orders of diffraction and selecting t such that n=p−t applies, with t being an integer larger zero, wherein not all of the p orders of diffraction are used in the image reconstruction and the number n of raw images evaluated is lower than the number p of the orders of diffraction characterizing the SIM illumination pattern.