CPC C12Q 1/6876 (2013.01) [C12N 15/1096 (2013.01); C12Q 1/686 (2013.01)] | 19 Claims |
1. A method for single cell analysis, the method comprising:
incubating a plurality of nucleic-acid-labelled, target-specific antibodies with a plurality of target cells to promote binding of the nucleic-acid-labelled, target-specific antibodies to target proteins expressed by the target cells;
washing the incubated target cells to remove unbound nucleic-acid-labelled, target-specific antibodies;
combining template particles and the washed target cells in a first fluid, wherein each template particle comprises first capture probes comprising capture sequences and second capture probes comprising template-switching oligos (TSOs);
adding a second fluid to the first fluid;
shearing the fluids to generate a plurality of monodisperse droplets simultaneously that contain a single one of the template particles and a single one of the target cells;
amplifying and sequencing nucleic acid labels from the nucleic-acid-labelled, target-specific antibodies to identify target proteins expressed by the target cells.
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