US 11,866,782 B2
Multi-omic analysis in monodisperse droplets
Sepehr Kiani, Watertown, MA (US)
Assigned to Fluent Biosciences Inc., Watertown, MA (US)
Filed by Fluent Biosciences Inc., Watertown, MA (US)
Filed on Mar. 16, 2021, as Appl. No. 17/202,783.
Claims priority of provisional application 62/990,368, filed on Mar. 16, 2020.
Prior Publication US 2021/0332432 A1, Oct. 28, 2021
Int. Cl. C12Q 1/6876 (2018.01); C12N 15/10 (2006.01); C12Q 1/686 (2018.01)
CPC C12Q 1/6876 (2013.01) [C12N 15/1096 (2013.01); C12Q 1/686 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A method for single cell analysis, the method comprising:
incubating a plurality of nucleic-acid-labelled, target-specific antibodies with a plurality of target cells to promote binding of the nucleic-acid-labelled, target-specific antibodies to target proteins expressed by the target cells;
washing the incubated target cells to remove unbound nucleic-acid-labelled, target-specific antibodies;
combining template particles and the washed target cells in a first fluid, wherein each template particle comprises first capture probes comprising capture sequences and second capture probes comprising template-switching oligos (TSOs);
adding a second fluid to the first fluid;
shearing the fluids to generate a plurality of monodisperse droplets simultaneously that contain a single one of the template particles and a single one of the target cells;
amplifying and sequencing nucleic acid labels from the nucleic-acid-labelled, target-specific antibodies to identify target proteins expressed by the target cells.