US 11,866,776 B2
Methods involving nucleic acid analysis of milk
Michel Georges, Liège (BE); Wouter Coppieters, Liège (BE); and Latifa Karim, Liège (BE)
Assigned to GESVAL S.A., Liège (BE)
Appl. No. 17/040,461
Filed by Université de Liège, Liège (BE)
PCT Filed Mar. 26, 2019, PCT No. PCT/EP2019/057628
§ 371(c)(1), (2) Date Sep. 22, 2020,
PCT Pub. No. WO2019/185654, PCT Pub. Date Oct. 3, 2019.
Claims priority of application No. 18164063 (EP), filed on Mar. 26, 2018.
Prior Publication US 2021/0024995 A1, Jan. 28, 2021
Int. Cl. C12Q 1/6869 (2018.01); C12Q 1/6827 (2018.01); C12Q 1/6888 (2018.01)
CPC C12Q 1/6869 (2013.01) [C12Q 1/6827 (2013.01); C12Q 1/6888 (2013.01); C12Q 2600/124 (2013.01); C12Q 2600/156 (2013.01)] 23 Claims
OG exemplary drawing
 
1. A method for determining the proportion or quantity of DNA contributed by individual animals to a volume of milk collected from at least 100 individual animals, the method comprising the steps of:
a) allele sampling for a plurality of DNA sequence polymorphisms in DNA extracted from a sample of the volume of milk by shallow whole genome sequencing (SWGS), wherein the sequencing depth of the SWGS is 10 or less and wherein the plurality of DNA sequence polymorphisms comprises at least 1×105 DNA sequence polymorphisms;
b) determining the proportion or quantity of DNA contributed by the individual animals to the volume of milk, based on the allele sampling of said DNA sequence polymorphisms from step a), and allelic data for said DNA sequence polymorphisms in the individual animals.