	US 11,866,765 B2
	Composition for improving molecular barcoding efficiency and use thereof
Dong Hyun Park, Chuncheon-si (KR); Jong Suk Chung, Hwaseong-si (KR); Seung Ho Shin, Seoul (KR); Sol Yi Kim, Seongnam-si (KR); Hyeyeun Lim, Bucheon-si (KR); and Hyeong Been Park, Seoul (KR)
Assigned to GENINUS INC., Seoul (KR)
Filed by GENINUS INC., Seoul (KR)
Filed on Apr. 8, 2021, as Appl. No. 17/225,597.
Claims priority of application No. 10-2021-0026287 (KR), filed on Feb. 26, 2021.
Prior Publication US 2022/0275425 A1, Sep. 1, 2022
Int. Cl. C12P 19/34 (2006.01); C12Q 1/6806 (2018.01); C12N 15/10 (2006.01); C12Q 1/6855 (2018.01); C12Q 1/6869 (2018.01)

CPC C12Q 1/6806 (2013.01) [C12N 15/1065 (2013.01); C12Q 1/6855 (2013.01); C12Q 1/6869 (2013.01)]

11 Claims

1. A method for preparing a DNA library for nucleic acid sequencing, the method comprising:

ligating an adapter comprising a barcode sequence to both ends of a DNA fragment extracted and fragmented from a target sample;

separating the ligated DNA fragment into single strands; and

amplifying the single-stranded DNA fragment ligated with the adapter through a polymerase chain reaction by using a primer that recognizes the adapter,

wherein the barcode sequence has a length of 2 nt to 10 nt and is arranged to include the position of a first nucleotide in the adapter, on the basis of a terminal region of the adapter ligated with the DNA fragment, and

wherein the second nucleotide in the adaptor is either G or C.