US 11,866,762 B2
Methods of targeted antibiotic susceptibility testing
Samad Talebpour, Richmond Hill (CA); Aye Aye Khine, Thornhill (CA); Tino Alavie, Thornhill (CA); and Stephen Wesley Leonard, Unionville (CA)
Assigned to QVELLA CORPORATION, Richmond Hill (CA)
Filed by QVELLA CORPORATION, Richmond Hill (CA)
Filed on Apr. 26, 2021, as Appl. No. 17/240,093.
Application 17/240,093 is a continuation of application No. 16/280,842, filed on Feb. 20, 2019, granted, now 10,988,794.
Application 16/280,842 is a continuation of application No. 14/901,200, granted, now 10,233,483, issued on Mar. 19, 2019, previously published as PCT/CA2014/050634, filed on Jul. 3, 2014.
Claims priority of provisional application 61/842,827, filed on Jul. 3, 2013.
Prior Publication US 2021/0246483 A1, Aug. 12, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/18 (2006.01); C12Q 1/689 (2018.01)
CPC C12Q 1/18 (2013.01) [C12Q 1/689 (2013.01); C12Q 2600/106 (2013.01); C12Q 2600/158 (2013.01)] 14 Claims
 
1. A method of performing rapid antimicrobial susceptibility testing, the method comprising:
initiating blood culture by:
obtaining whole blood from a patient and contacting the whole blood with growth media, thereby obtaining a blood culture mixture; and
incubating the blood culture mixture under conditions suitable for promoting microbial growth;
after having initiated incubation of the blood culture mixture, but prior to a determination of positivity by conventional blood culture:
obtaining a first sample from the blood culture mixture;
separating microbial cells from the first sample to obtain a first suspension of microbial cells;
employing the first suspension of microbial cells to perform a microbial identification assay, the microbial identification assay identifying, from a panel of microbial cell types, a microbial cell type associated with microbial cells residing with the first sample;
obtaining a second sample from the blood culture mixture;
separating microbial cells from the second sample to obtain a second suspension of microbial cells; and
employing the second suspension of microbial cells to perform an antimicrobial susceptibility assay, the antimicrobial susceptibility assay determining a measure of the effectiveness of one or more antibiotics on microbial cells within the second sample, the one or more antibiotics being selected based on results from the microbial identification assay; and
providing results from the antimicrobial susceptibility assay prior to the determination of positivity by conventional blood culture.