	US 11,866,759 B1
	Method of producing calcium propionate by using Lactobacillus reuteri
Jianying Wang, Guangdong (CN); and Zongyao Kong, Guangdong (CN)
Assigned to SHENZHEN AGRECOE BIOTECHNOLOGY CO., LTD., Shenzhen (CN)
Filed by SHENZHEN AGRECOE BIOTECHNOLOGY CO., LTD., Guangdong (CN)
Filed on Apr. 19, 2023, as Appl. No. 18/302,911.
Claims priority of application No. 202210725513.3 (CN), filed on Jun. 24, 2022.
Int. Cl. C12P 7/52 (2006.01); C12N 9/04 (2006.01); C12N 1/20 (2006.01); C12N 15/01 (2006.01)

CPC C12P 7/52 (2013.01) [C12N 1/205 (2021.05); C12N 9/0006 (2013.01); C12N 15/01 (2013.01); C12Y 101/01001 (2013.01)]

5 Claims

1. A method of producing calcium propionate by using Lactobacillus reuteri, wherein the method comprises the following steps:

conducting a single base mutation on an alcohol dehydrogenase gene of the Lactobacillus reuteri by using a gene editing tool to obtain Lactobacillus reuteri having an inactivated alcohol dehydrogenase;

mutating the Lactobacillus reuteri having the inactivated alcohol dehydrogenase by an atmospheric room temperature plasma mutation technology to obtain a mutated Lactobacillus reuteri having an inactivated alcohol dehydrogenase;

mixing the mutated Lactobacillus reuteri having the inactivated alcohol dehydrogenase with 1,2-propanediol, then growing and reproducing the mutated Lactobacillus reuteri having the inactivated alcohol dehydrogenase, and then conducting culture of the mutated Lactobacillus reuteri having the inactivated alcohol dehydrogenase; and

after the culture, placing the mutated Lactobacillus reuteri having the inactivated alcohol dehydrogenase into a culture medium containing calcium ion for a fermentation culture, then obtaining the calcium propionate.