	US 11,866,482 B2
	System and method for homogenous GPCR phosphorylation and identification of beta-2 adrenergic receptor positive allosteric modulators
Robert J. Lefkowitz, Durham, NC (US); Seungkirl Ahn, Durham, NC (US); Biswaranjan Pani, Durham, NC (US); Alem W. Kahsai, Durham, NC (US); Laura Wingler, Durham, NC (US); and Dean Staus, Durham, NC (US)
Assigned to Duke University, Durham, NC (US)
Filed by Duke University, Durham, NC (US)
Filed on Feb. 7, 2019, as Appl. No. 16/269,877.
Claims priority of provisional application 62/627,678, filed on Feb. 7, 2018.
Claims priority of provisional application 62/627,680, filed on Feb. 7, 2018.
Prior Publication US 2019/0241642 A1, Aug. 8, 2019
Int. Cl. C07K 16/28 (2006.01); C07K 14/72 (2006.01); C07K 14/705 (2006.01); C07K 14/47 (2006.01); G01N 33/68 (2006.01); C07K 19/00 (2006.01)

CPC C07K 14/723 (2013.01) [C07K 14/4703 (2013.01); C07K 14/70567 (2013.01); C07K 14/70571 (2013.01); C07K 19/00 (2013.01); G01N 33/6872 (2013.01); C07K 2319/00 (2013.01); C07K 2319/70 (2013.01); G01N 2333/726 (2013.01); G01N 2500/02 (2013.01); G01N 2500/04 (2013.01); G01N 2500/10 (2013.01)]

6 Claims

1. A complex comprising:

(i) a chimeric G protein-coupled receptor (GPCR) comprising the amino acid sequence LPETGGG (SEQ ID NO: 1) located within the C-terminus of the GPCR and a synthetic phosphopeptide ligated to SEQ ID NO: 1, wherein the synthetic phosphopeptide is derived from the C-terminus of a vasopressin-2-receptor (V2R); and

(ii) a β-arrestin (βarr) protein bound to the C-terminus of the GPCR.