US 11,865,211 B2
Nanoparticle complex showing improved cellular uptake through surface modification using lipid and manufacturing method therefor
Hyun Cheol Kim, Seoul (KR); and Do Yeon Kim, Seoul (KR)
Assigned to INSBIOPHARM CO., LTD.
Appl. No. 17/056,402
Filed by SOGANG UNIVERSITY RESEARCH FOUNDATION, Seoul (KR)
PCT Filed Sep. 4, 2018, PCT No. PCT/KR2018/010309
§ 371(c)(1), (2) Date Nov. 17, 2020,
PCT Pub. No. WO2019/231051, PCT Pub. Date Dec. 5, 2019.
Claims priority of application No. 10-2018-0063163 (KR), filed on Jun. 1, 2018.
Prior Publication US 2021/0308050 A1, Oct. 7, 2021
Int. Cl. A61K 9/127 (2006.01); A61K 9/50 (2006.01); A61K 9/51 (2006.01); A61K 31/704 (2006.01); A61K 31/7105 (2006.01)
CPC A61K 9/1271 (2013.01) [A61K 9/1277 (2013.01); A61K 9/5015 (2013.01); A61K 9/5089 (2013.01); A61K 9/5192 (2013.01); A61K 31/704 (2013.01); A61K 31/7105 (2013.01)] 2 Claims
OG exemplary drawing
 
1. A method of manufacturing a nanoparticle complex, the method comprising:
a nanoparticle-forming step of forming nanoparticle including a first reactive group;
a liposome-forming step of forming phospholipid-based liposome having a micro size including a second reactive group for bonding chemically to the first reactive group;
a nanoparticle complex forming step of mixing the nanoparticle and the liposome so that the first reactive group and the second reactive group are bonded to each other, thus forming the nanoparticle complex in which the nanoparticles are bonded to outer sides of the liposome; and
a crushing formation step of applying mechanical force to the nanoparticle complex formed so that the liposome is crushed to form a lipid structure bonded to a portion of an outer surface of the nanoparticle, thus manufacturing the nanoparticle complex,
wherein the nanoparticle is taken into cells to be used for treatment of diseases, and the lipid structure improves a cellular uptake of the nanoparticle,
wherein the nanoparticle has a diameter of 100 to 300 nm, and the lipid structure has a length of 50 to 300 nm and a width of 3 to 20 nm.