US 10,890,557 B2
Electrochemical biosensor
In Jun Yoon, Seoul (KR); Jae Ho Shin, Seoul (KR); Yeon Ho Jung, Gyeonggi-do (KR); Geun Sig Cha, Seoul (KR); and Hakhyun Nam, Seoul (KR)
Assigned to I-SENS, INC., Seoul (KR)
Appl. No. 15/533,333
Filed by I-SENS, INC., Seoul (KR)
PCT Filed Dec. 31, 2015, PCT No. PCT/KR2015/014582
§ 371(c)(1), (2) Date Jun. 5, 2017,
PCT Pub. No. WO2016/108671, PCT Pub. Date Jul. 7, 2016.
Claims priority of application No. 10-2014-0195173 (KR), filed on Dec. 31, 2014.
Prior Publication US 2017/0350850 A1, Dec. 7, 2017
Int. Cl. G01N 27/416 (2006.01); G01N 27/327 (2006.01); C12M 1/40 (2006.01); G01N 33/66 (2006.01); C07D 401/04 (2006.01); C07F 15/00 (2006.01); C12Q 1/00 (2006.01)
CPC G01N 27/416 (2013.01) [C07D 401/04 (2013.01); C07F 15/002 (2013.01); C12M 1/40 (2013.01); C12Q 1/004 (2013.01); C12Q 1/006 (2013.01); G01N 27/327 (2013.01); G01N 33/66 (2013.01)] 18 Claims
OG exemplary drawing
 
1. An electrochemical biosensor, comprising
an electrode substrate, and
an osmium complex represented by Chemical formula 1,
Os(A)mXn,
and an oxidizing agent,
wherein, A is represented by Chemical formula 2,

OG Complex Work Unit Drawing
wherein X is a halogen, m is an integer of 1 to 3, n is an integer of 0 to 4, and a sum of m and n is an integer of 3 to 5, or a salt thereof;
an enzyme being capable of oxidizing and reducing a target substance in a liquid biological sample, wherein said complex and said enzyme are immobilized on the electrode substrate; and
at least one selected from the group consisting of surfactants, water-soluble polymers, quaternary ammonium salts, fatty acids, and thickening agents,
wherein the oxidizing agent is in a molar ratio of 0.1 to 10, based on 1 mole of osmium complex or its salt.