US 10,889,868 B2
Exosomal biomarkers diagnostic of tuberculosis
Jeffrey S. Schorey, Granger, IN (US); Prachi Pratap Singh, South Bend, IN (US); and Yong Cheng, South Bend, IN (US)
Assigned to University of Notre Dame du Lac, South Bend, IN (US)
Filed by University of Notre Dame du Lac, South Bend, IN (US)
Filed on Nov. 21, 2019, as Appl. No. 16/690,678.
Application 16/690,678 is a continuation of application No. 15/449,324, filed on Mar. 3, 2017, granted, now 10,526,665.
Claims priority of provisional application 62/303,631, filed on Mar. 4, 2016.
Prior Publication US 2020/0102601 A1, Apr. 2, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/689 (2018.01); C12Q 1/6883 (2018.01)
CPC C12Q 1/689 (2013.01) [C12Q 1/6883 (2013.01); C12Q 2600/158 (2013.01)] 18 Claims
 
1. A method for identifying an active M. tuberculosis infection in a subject comprising:
isolating extracellular vesicles from bodily fluid of a subject, wherein the extracellular vesicles contain a plurality of RNA;
extracting at least a portion of the RNA from the extracellular vesicle; and
analyzing the RNA for the presence of one or more RNA that are indicative of an active M. tuberculosis infection; wherein the RNA that are indicative of an active M. tuberculosis infection are selected from the group consisting of RV1821, RV1842c, RV3894c, RV0453, RV1629, RV0170, RV0668, RV0740, RV0288, RV1344, RV0968, RV1942c, RV0664, RV0190, RV1757c, RV1369c, RV3809c, RV3533, RV0243, RV1101c, RV2796, and RV2024c, thereby determining the presence or absence of an active M. tuberculosis infection in the subject.