US 10,889,859 B2
Transposition of native chromatin for personal epigenomics
Paul Giresi, Palo Alto, CA (US); Jason D. Buenrostro, Redwood City, CA (US); Howard Y. Chang, Stanford, CA (US); and William J. Greenleaf, Menlo Park, CA (US)
Assigned to The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US)
Filed by The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US)
Filed on Jul. 1, 2020, as Appl. No. 16/918,924.
Application 16/918,924 is a continuation of application No. 16/418,796, filed on May 21, 2019.
Application 16/418,796 is a continuation of application No. 16/160,719, filed on Oct. 15, 2018, granted, now 10,337,062, issued on Jul. 2, 2019.
Application 16/160,719 is a continuation of application No. 16/043,874, filed on Jul. 24, 2018, granted, now 10,150,995, issued on Dec. 11, 2018.
Application 16/043,874 is a continuation of application No. 14/784,250, granted, now 10,059,989, issued on Aug. 28, 2018, previously published as PCT/US2014/038825, filed on May 20, 2014.
Claims priority of provisional application 61/826,728, filed on May 23, 2013.
Prior Publication US 2020/0332359 A1, Oct. 22, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6874 (2018.01); C12Q 1/6869 (2018.01); G16B 30/00 (2019.01); G16H 50/20 (2018.01); G16B 20/00 (2019.01); C12Q 1/6806 (2018.01)
CPC C12Q 1/6874 (2013.01) [C12Q 1/6806 (2013.01); C12Q 1/6869 (2013.01); G16B 20/00 (2019.02); G16B 30/00 (2019.02); G16H 50/20 (2018.01); C12Q 2521/301 (2013.01); C12Q 2537/164 (2013.01)] 30 Claims
 
1. A method for analyzing chromatin, comprising:
(a) isolating a plurality of cell nuclei from a cell sample;
(b) contacting chromatin from a nucleus of said plurality of cell nuclei with an insertional enzyme complex to produce a plurality of tagged fragments of genomic deoxyribonucleic acid (DNA) in said nucleus;
(c) providing said nucleus into a nucleic acid reaction mixture, wherein said nucleic acid reaction mixture comprises at most a single nucleus; and
(d) performing one or more nucleic acid reactions on a tagged fragment of said plurality of tagged fragments.