US 10,889,848 B2
Process for determining viability of test microorganisms of biological indicator and sterilization detection device for determining same
Phillip P. Franciskovich, Concord, OH (US); Kathleen A. Fix, Willoughby, OH (US); and Michael A. Centanni, Parma, OH (US)
Assigned to American Sterilizer Company, Mentor, OH (US)
Filed by American Sterilizer Company, Mentor, OH (US)
Filed on Sep. 8, 2017, as Appl. No. 15/699,240.
Claims priority of provisional application 62/532,520, filed on Jul. 14, 2017.
Prior Publication US 2019/0017093 A1, Jan. 17, 2019
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/22 (2006.01); C12M 1/12 (2006.01); G01N 33/497 (2006.01)
CPC C12Q 1/22 (2013.01) [C12M 37/06 (2013.01); G01N 2033/4977 (2013.01)] 22 Claims
OG exemplary drawing
 
1. A process for determining the viability of a biological indicator, the process comprising:
subjecting the biological indicator to a sterilization process in which the biological indicator is exposed to a sterilization medium, the biological indicator comprising test microorganisms on a carrier;
subsequent to the sterilization process, exposing the biological indicator to a viability detection medium comprising hydrogen peroxide, the exposing the biological indicator to the detection medium comprising the hydrogen peroxide producing a gaseous reaction product when one or more of the test microorganisms are viable; and
detecting with a sensing device the presence or absence of the gaseous reaction product produced by the biological indicator combined with the viability detection medium, the sensing device comprising a capacitive sensor, wherein the presence of the gaseous reaction product indicates the presence of viable test microorganisms and the absence of the gaseous reaction product indicates the absence of viable test microorganisms.