US 10,889,840 B2
Conversion of methylglyoxal into hydroxyacetone using novel enzymes and applications thereof
Laetitia Faure, Les Martres de Veyre (FR); Gwenaëlle Corre, Saint Beauzire (FR); Céline Raynaud, Saint Beauzire (FR); and Laurence Dumon-Seignovert, Pont du Chateau (FR)
Assigned to METABOLIC EXPLORER, Saint Beauzire (FR)
Appl. No. 16/465,560
Filed by METABOLIC EXPLORER, Saint Beauzire (FR)
PCT Filed Dec. 29, 2017, PCT No. PCT/EP2017/084841
§ 371(c)(1), (2) Date May 30, 2019,
PCT Pub. No. WO2018/122388, PCT Pub. Date Jul. 5, 2018.
Claims priority of application No. 16306848 (EP), filed on Dec. 29, 2016.
Prior Publication US 2020/0181652 A1, Jun. 11, 2020
Int. Cl. C12N 15/70 (2006.01); C12P 7/18 (2006.01); C12N 9/04 (2006.01); C12N 9/02 (2006.01); C12N 9/88 (2006.01); C12P 7/28 (2006.01)
CPC C12P 7/18 (2013.01) [C12N 9/0006 (2013.01); C12N 9/0008 (2013.01); C12N 9/0036 (2013.01); C12N 9/88 (2013.01); C12P 7/28 (2013.01); C12Y 101/01072 (2013.01); C12Y 101/01283 (2013.01); C12Y 102/01013 (2013.01); C12Y 402/01136 (2015.07); C12Y 402/03003 (2013.01); C12Y 404/01005 (2013.01)] 19 Claims
 
1. A method for the fermentative conversion of methylglyoxal into hydroxyacetone, comprising the step of expressing, in a microorganism, at least one methylglyoxal reductase having a catalytic efficiency kcat/Km equal or superior to 5 mM−1s−1 wherein said methylglyoxal reductase is selected from the group consisting of YjgB having the sequence of SEQ ID NO: 1, YjgB* (N240Y) having the sequence of SEQ ID NO: 9, YigB*(I165V) having the sequence of SEQ ID NO: 125 and YigB*(Q39R/I165V/A296V) having the sequence of SEQ ID NO: 127.