US 10,888,109 B2
Nitrite-degrading and TSNA-degrading bacteria and methods of making and using
Ujwala Warek, Chester, VA (US); Mark T. Nielsen, Nicholasville, KY (US); Dongmei Xu, Glen Allen, VA (US); Mingwu Cui, Lexington, KY (US); Xue Luo, Richmond, VA (US); Xiaohong Jin, Glen Allen, VA (US); Elisabeth Miller, Chesterfield, VA (US); and Kwang Choi, Glen Allen, VA (US)
Assigned to Altria Client Services LLC, Richamond, VA (US)
Filed by Altria Client Services LLC, Richmond, VA (US)
Filed on Jan. 15, 2019, as Appl. No. 16/248,300.
Application 16/248,300 is a continuation of application No. 14/206,666, filed on Mar. 12, 2014, granted, now 10,226,064.
Claims priority of provisional application 61/794,854, filed on Mar. 15, 2013.
Prior Publication US 2019/0200668 A1, Jul. 4, 2019
This patent is subject to a terminal disclaimer.
Int. Cl. A24B 15/24 (2006.01); A24B 15/20 (2006.01); A24B 15/12 (2006.01); C12N 1/20 (2006.01)
CPC A24B 15/245 (2013.01) [A24B 15/12 (2013.01); A24B 15/20 (2013.01); C12N 1/20 (2013.01)] 20 Claims
1. A method of reducing the amount of at least one tobacco-specific nitrosamine (TSNA) in a composition comprising tobacco, comprising contacting said composition with an isolated bacterial strain of Myroides odoratus comprising a 16S rDNA sequence at least 98% identical to SEQ ID NO: 1 under conditions in which the amount of said at least one TSNA is reduced in said composition, wherein said conditions comprise (a) a moisture content from about 45% to about 75% by weight of said tobacco; (b) a temperature from about 20° C. to about 45° C.; (c) a pH of about 5 to about 8; or (d) any combination of (a), (b), and (c), thereby producing a reduced-TSNA composition.