US 10,888,056 B2
Tomato fruit having increased firmness
Charles James Baxter, Grimoldby near Louth (GB); Natalie Helene Chapman, Loughborough (GB); Ian Puddephat, Reading (GB); and Graham Barron Seymour, Loughborough (GB)
Assigned to Syngenta Participations AG, Basel (CH)
Filed by Syngenta Participations AG, Basel (CH)
Filed on Jun. 13, 2019, as Appl. No. 16/439,838.
Application 16/439,838 is a division of application No. 14/637,084, filed on Mar. 3, 2015, granted, now 10,362,741.
Application 14/637,084 is a division of application No. 13/500,149, granted, now 8,987,549, issued on Mar. 24, 2015, previously published as PCT/EP2010/065575, filed on Oct. 15, 2010.
Claims priority of application No. 09174072 (EP), filed on Oct. 26, 2009.
Prior Publication US 2019/0313594 A1, Oct. 17, 2019
Int. Cl. A01H 1/00 (2006.01); A01H 5/08 (2018.01); C12N 15/82 (2006.01); A01H 6/82 (2018.01)
CPC A01H 5/08 (2013.01) [A01H 1/00 (2013.01); A01H 6/825 (2018.05); C12N 15/8242 (2013.01)] 4 Claims
 
1. A method for selecting a cultivated Solanum lycopersicum plant that provides fruit with increased firmness of the inner pericarp as compared with fruit from a control Solanum lycopersicum plant, the method comprising:
a) detecting in the cultivated Solanum lycopersicum plant the presence of QTL2 on chromosome 2, and
b) selecting a cultivated Solanum lycopersicum plant that includes the presence of said QTL2, thereby selecting a cultivated Solanum lycopersicum plant that provides fruit with increased firmness of the inner pericarp as compared with fruit from a control Solanum lycopersicum plant;
wherein detecting the presence of said QTL2 in the cultivated Solanum lycopersicum plant comprises:
i) providing a sample of genomic DNA from the cultivated Solanum lycopersicum plant, and
ii) detecting in the sample of genomic DNA at least one DNA marker including HOX7A, wherein HOX7A can be detected by a forward primer of SEQ ID NO: 7 and a reverse primer of SEQ ID NO: 8; and
wherein the increased fruit firmness of the inner pericarp is from between 1.2 to 2 times greater at the harvesting stage than that of fruit from the control Solanum lycopersicum plant.