| CPC G16B 5/30 (2019.02) [C12N 15/63 (2013.01); G16B 5/00 (2019.02); G16B 20/00 (2019.02); G16B 25/00 (2019.02)] | 20 Claims |
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1. A method for determining an intrinsic property of a DNA component involved in transcription of a target signal, the method comprising:
accessing a plurality of measurements obtained over a time period from a cell culture, wherein each cell in the cell culture comprises the DNA component, and wherein the plurality of measurements comprises a first measurement relating to an amount of the target signal in the cell culture over the time period and a second measurement relating to a density of the cell culture of the time period;
estimating parameters quantifying the intrinsic property of the DNA component by:
embedding the estimated parameters within a mathematical model that describes a production of the target signal over the time period; and
minimizing a measure of a difference between outputs of the mathematical model and the first measurement;
estimating parameter values for a second mathematical model that describes cell culture density over time by minimizing a measure of a difference between an output of the second mathematical model and the second measurement; and
based on the estimated parameters and the estimated parameter values, identifying the intrinsic property.
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