US 12,189,105 B2
Interferometric scattering microscopy
Philipp Kukura, Oxford (GB); Alexander Weigel, Garching bei Munich (DE); and Justin Benesch, Oxford (GB)
Assigned to Oxford University Innovation Limited, Oxford (GB)
Filed by Oxford University Innovation Limited, Oxford (GB)
Filed on May 8, 2023, as Appl. No. 18/144,372.
Application 18/144,372 is a continuation of application No. 16/992,350, filed on Aug. 13, 2020, abandoned.
Application 16/992,350 is a continuation of application No. 16/107,551, filed on Aug. 21, 2018, granted, now 10,775,597, issued on Sep. 15, 2020.
Application 16/107,551 is a continuation in part of application No. PCT/GB2017/052070, filed on Jul. 13, 2017.
Claims priority of application No. 1612182 (GB), filed on Jul. 13, 2016.
Prior Publication US 2023/0359009 A1, Nov. 9, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. G02B 21/00 (2006.01); G01G 9/00 (2006.01); G01N 15/1434 (2024.01); G02B 21/08 (2006.01); G02B 21/14 (2006.01); G02B 21/26 (2006.01); G02B 21/36 (2006.01)
CPC G02B 21/0032 (2013.01) [G01G 9/005 (2013.01); G01N 15/1434 (2013.01); G02B 21/0004 (2013.01); G02B 21/008 (2013.01); G02B 21/082 (2013.01); G02B 21/14 (2013.01); G02B 21/26 (2013.01); G02B 21/36 (2013.01); G02B 21/361 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method of characterizing interactions and/or assembly of individual biomolecules using an interferometric light scattering microscope, said interferometric light scattering microscope comprising:
a sample holder for holding a sample in a sample location;
an illumination source arranged to provide illuminating light;
a detector;
an optical system being arranged to direct illuminating light onto the sample location and being arranged to collect output light in reflection, the output light comprising both light scattered from the sample location and illuminating light reflected from the sample location, and to direct the output light to the detector; and
a spatial filter positioned to filter the output light, the spatial filter being arranged to pass output light but with a reduction in intensity that is greater within a predetermined numerical aperture than at larger numerical apertures.