(a) providing the peptide coupled to the solid support, the peptide coupled to the solid support such that a N-terminal amino acid residue of the peptide is not directly coupled to the solid support and is exposed to reaction conditions;

(b) providing a chemically-reactive conjugate, the chemically-reactive conjugate comprising: (x) a cycle tag comprising a cycle nucleic acid associated with a cycle number, (y) a reactive moiety for binding the N-terminal amino acid residue of the peptide, and (z) an immobilizing moiety for immobilization to the solid support;

(c) contacting the peptide with the chemically-reactive conjugate, thereby coupling the chemically-reactive conjugate to the N-terminal amino acid of the peptide to form a conjugate complex;

(d) immobilizing the conjugate complex to the solid support via the immobilizing moiety;

(e) cleaving and thereby separating the N-terminal amino acid residue from the peptide, thereby providing an immobilized single-amino acid complex, the immobilized single-amino acid complex comprising the cleaved and separated N-terminal amino acid residue and the cycle nucleic acid;

(f) contacting the immobilized single-amino acid complex with a binding agent, the binding agent comprising: a binding moiety for preferentially binding to the immobilized single-amino acid complex, and a recode tag comprising a recode nucleic acid corresponding with the binding agent, thereby forming an affinity complex, the affinity complex comprising the immobilized single-amino acid complex and the binding agent, and thereby bringing the cycle tag into proximity with the recode tag within the affinity complex;

(g) combining sequence information of the recode nucleic acid and the cycle nucleic acid of the immobilized single-amino acid complex to generate a recode block; and

(j) obtaining sequence information of the recode block.