US 12,188,935 B2
Method for the evaluation of antiretroviral therapy (ART) effectiveness in HIV-1 CD4+CD89+ cellular reservoirs
Monsef Benkirane, Saint Gely du Fesc (FR); Gael Petitjean, Montpellier (FR); and Benjamin Descours, Montpellier (FR)
Assigned to CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, Paris (FR); and UNIVERSITÉ DE MONTPELLIER, Montpellier (FR)
Filed by CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, Paris (FR); and UNIVERSITÉ DE MONTPELLIER, Montpellier (FR)
Filed on May 7, 2021, as Appl. No. 17/314,255.
Application 17/314,255 is a continuation in part of application No. 16/337,003, granted, now 11,041,858, previously published as PCT/IB2017/056055, filed on Sep. 30, 2017.
Claims priority of application No. 16/59440 (FR), filed on Sep. 30, 2016; and application No. 17/52126 (FR), filed on Mar. 15, 2017.
Prior Publication US 2021/0325391 A1, Oct. 21, 2021
Int. Cl. G01N 33/569 (2006.01)
CPC G01N 33/56988 (2013.01) [G01N 33/56972 (2013.01); G01N 2333/16 (2013.01); G01N 2333/70514 (2013.01); G01N 2333/70517 (2013.01); G01N 2333/70535 (2013.01); G01N 2333/70589 (2013.01); G01N 2500/10 (2013.01); G01N 2800/52 (2013.01)] 8 Claims
 
1. A method for the evaluation of the efficacy of an antiretroviral therapy (ART) treatment aiming to eradicate a cellular reservoirs of HIV-1 associated with CD4 +CD89+ T-cells in an HIV-1 subject who has received or is currently undergoing the ART treatment, said method comprising:
detecting CD4+CD89+ T-cells using detection reagents that are specific to those cell markers in peripheral blood mononuclear cells (PBMCs) isolated from a biological sample obtained from the HIV-1 subject;
detecting HIV-1 viral DNA in said CD4+ CD89+ T-cells;
isolating detected CD4+ CD89+ T-cells, said CD4+ CD89+ T-cells being associated with the cellular reservoirs of HIV-1;
quantifying the isolated CD4+ CD89+ T-cells in the biological sample obtained from the HIV-subject using a detection reagent specific to the differentiation marker CD89, NI being the number of isolated CD4+ CD89+ T-cells quantified in the biological sample obtained from the HIV-subject; and
wherein the ART treatment:
is efficient to eradicate the cellular reservoirs of HIV-1 associated with the CD4+ CD89+ T-cells when the number NI is equal to 0, and
is not efficient to eradicate the cellular reservoirs of HIV-1 associated with the CD4+ CD89+ T-cells when the number NI is not equal to 0.