US 12,188,933 B2
Tools for detecting cocoa swollen shoot virus coat protein antigen
Richard Luxton, Bristol (GB); Janice Kiely, Bristol (GB); Joel Allainguillaume, Bristol (GB); and Jacqueline Mary Barnett, Bristol (GB)
Assigned to University of the West of England, Bristol, Bristol (GB)
Appl. No. 17/289,850
Filed by University of the West of England, Bristol, Bristol (GB)
PCT Filed Oct. 29, 2019, PCT No. PCT/GB2019/053056
§ 371(c)(1), (2) Date Apr. 29, 2021,
PCT Pub. No. WO2020/089607, PCT Pub. Date May 7, 2020.
Claims priority of application No. 1817721 (GB), filed on Oct. 30, 2018; and application No. 1908311 (GB), filed on Jun. 11, 2019.
Prior Publication US 2021/0405050 A1, Dec. 30, 2021
Int. Cl. G01N 33/569 (2006.01); G01N 33/543 (2006.01)
CPC G01N 33/56983 (2013.01) [G01N 33/54366 (2013.01); G01N 2333/01 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method for detecting Cocoa Swollen Shoot Virus (CSSV) using a porous membrane based sensor, the sensor comprising at least one recombinant CSSV coat protein antigen having at least 80% sequence identity to the amino acid sequence of SEQ ID NO: 2 or 4, the method comprising the steps of:
(i) contacting cocoa plant material with a labelled binding reagent that specifically binds to the recombinant coat protein antigen to produce a mixture comprising the plant material and binding reagent; and
(ii) contacting the mixture with the membrane based sensor,
wherein the binding reagent is a monoclonal antibody comprising:
a) a heavy chain CDR1 of SEQ ID NO:17, a heavy chain CDR2 of SEQ ID NO: 18, a heavy chain CDR3 of SEQ ID NO: 19, a light chain CDR1 of SEQ ID NO:20, a light chain CDR2 of SEQ ID NO:21, and a light chain CDR3 of SEQ ID NO:22; or
b) a heavy chain CDR1 of SEQ ID NO:26, a heavy chain CDR2 of SEQ ID NO: 27, a heavy chain CDR3 of SEQ ID NO:28, a light chain CDR1 of SEQ ID NO:29, a light chain CDR2 of SEQ ID NO:30, and a light chain CDR3 of SEQ ID NO:31.