US 12,188,928 B2
Competitive immunochromatographic analysis method and immunochromatographic sensor
Kazushi Misawa, Kawasaki (JP); Daniel Citterio, Kawasaki (JP); Hiroki Yamazaki, Yokohama (JP); and Tomohiro Yamamoto, Yokohama (JP)
Assigned to Techno Medica Co., Ltd., Kanagawa (JP); and Keio University, Tokyo (JP)
Appl. No. 17/055,672
Filed by TECHNO MEDICA CO., LTD., Yokohama (JP); and KEIO UNIVERSITY, Tokyo (JP)
PCT Filed May 16, 2019, PCT No. PCT/JP2019/019587
§ 371(c)(1), (2) Date Nov. 16, 2020,
PCT Pub. No. WO2019/221250, PCT Pub. Date Nov. 21, 2019.
Claims priority of application No. 2018-095639 (JP), filed on May 17, 2018.
Prior Publication US 2021/0199649 A1, Jul. 1, 2021
Int. Cl. G01N 33/543 (2006.01)
CPC G01N 33/54306 (2013.01) 8 Claims
OG exemplary drawing
 
1. Competitive immunochromatographic analysis method characterized in that the method comprises:
supplying a mixed solution in which a test solution containing a substance to be measured A and a labeled antibody B are mixed to one or more judgement parts, wherein said judgement parts are formed by immobilizing a text antibody C which specifically reacts with the labeled antibody B specifically reacting with the substance to be measured A on a membrane carrier in the form of character(s) or figure(s) and immobilizing a mask antigen D that is the same antigen as the substance to be measured A on the membrane carrier so as to surround said text antibody C,
competitively binding the mixed solution having the labeled antibody B and the substance to be measured A to the mask antigen D and the text antibody C on the judgement parts, and
judging the concentration of the substance to be measured A based on the signal from the labeled antibody B bound to the text antibody C and the mask antigen D.