US 12,188,899 B2
Sensing compositions, methods, and devices for the detection of molecules using a nanopore device
Denise Ann McGrath, Scotts Valley, CA (US); Tyler Daniel Shropshire, Santa Cruz, CA (US); and Andrew Martin Smith, Santa Cruz, CA (US)
Assigned to Oxford Nanopore Technologies plc, Oxford (GB)
Appl. No. 17/274,159
Filed by Ontera Inc., Santa Cruz, CA (US)
PCT Filed Sep. 6, 2019, PCT No. PCT/US2019/050087
§ 371(c)(1), (2) Date Mar. 5, 2021,
PCT Pub. No. WO2020/068400, PCT Pub. Date Apr. 2, 2020.
Claims priority of provisional application 62/728,584, filed on Sep. 7, 2018.
Claims priority of provisional application 62/728,672, filed on Sep. 7, 2018.
Prior Publication US 2021/0310988 A1, Oct. 7, 2021
Int. Cl. G01N 27/327 (2006.01); C12Q 1/6806 (2018.01); G01N 27/447 (2006.01)
CPC G01N 27/44747 (2013.01) [C12Q 1/6806 (2013.01)] 42 Claims
OG exemplary drawing
 
1. A method for detecting or characterization of a polynucleotide in a sample within a nanopore device comprising:
a. contacting a sample suspected of comprising a polynucleotide with a sensing solution comprising an effective amount of a polyether agent of Formula (I):

OG Complex Work Unit Chemistry
wherein:
m is 1-3;
n is 1-30;
each R1 is independently H or methyl; and
R2 and R3 are each independently H or C(1-6)alkyl,
wherein the sensing solution is contacting a nanoporous membrane of a nanopore device, wherein the nanoporous membrane separates the space of the nanopore device into a cis volume and a trans volume;
b. applying a voltage across a nanopore of the nanoporous membrane thereby inducing translocation of the polynucleotide through the nanoporous membrane; and
c. detecting a current during the translocation of the polynucleotide in the nanopore device; and
(d) characterizing the polynucleotide as having a length of less than 500 base pairs (bps), wherein the nanopore device containing the sensing solution allows for discrimination of the polynucleotide by size.