US 12,188,862 B2
Harmonics microscope for measuring glycated hemoglobin fraction of single red blood cell
Chi-Kuang Sun, Taipei (TW); and Xu-hao Ye, Taipei (TW)
Assigned to National Taiwan University, Taipei (TW)
Filed by National Taiwan University, Taipei (TW)
Filed on Feb. 24, 2023, as Appl. No. 18/113,704.
Claims priority of application No. 112104082 (TW), filed on Feb. 6, 2023.
Prior Publication US 2024/0264073 A1, Aug. 8, 2024
Int. Cl. G01N 21/31 (2006.01); G01N 33/49 (2006.01); G02B 21/00 (2006.01); G02B 27/10 (2006.01); G02B 27/14 (2006.01)
CPC G01N 21/3103 (2013.01) [G01N 33/49 (2013.01); G02B 21/0032 (2013.01); G02B 21/0064 (2013.01); G02B 27/1013 (2013.01); G02B 27/141 (2013.01); G02B 27/145 (2013.01)] 7 Claims
OG exemplary drawing
 
1. A harmonics microscope for measuring a glycated hemoglobin fraction (HbA1c fraction) of a single red blood cell (RBC),
said harmonics microscope measuring said HbA1c fraction of a single RBC in a trace blood sample,
said harmonics microscope measuring an HbA1c fraction of said single RBC through a non-invasive way while said single RBC flows in a human epidermal microvessel,
said harmonics microscope comprising
a laser device,
said laser device emitting laser beams of different wavelengths,
each of said laser beams having a central wavelength and a frequency to specifically optimize optical characteristics of glycated hemoglobin to measure said HbA1c fraction;
an upright microscope,
said upright microscope receiving said laser beams of different wavelengths from said laser device,
said upright microscope projecting said laser beams of different wavelengths onto a single RBC to obtain harmonic observing beams of different wavelengths;
a light splitter,
said light splitter receiving said harmonic observing beams of different wavelengths from said upright microscope,
said light splitter dividing said harmonic observing beams into a first third harmonic generation (THG) segment, a second THG segment, a third THG segment, and a second harmonic generation (SHG) segment;
an optical detector,
said optical detector receiving said first THG segment, said second THG segment, said third THG segment, and said SHG segment from said light splitter,
said optical detector detecting said first THG segment, said second THG segment, said third THG segment, and said SHG segment to be converted into a first THG image signal, a second THG image signal, a third THG image signal, and an SHG image signal; and
a mainframe,
said mainframe receiving said first THG image signal, said second THG image signal, said third THG image signal, and said SHG image signal from said optical detector,
said mainframe integrating said first THG image signal, said second THG image signal, said third THG image signal, and said SHG image signal into a multispectral image,
wherein said mainframe has an image processing software; and, after said image processing software receives said multispectral image through said mainframe, said HbA1c fraction of said single RBC is measured with said multispectral image,
wherein said first THG segment characterizes signals provided by hemoglobin;
said second THG segment characterizes signals provided by glycated hemoglobin; and
said HbA1c fraction is a ratio of an electrical signal strength of said second THG segment to that of said first THG segment.