US 12,188,856 B2
Particle analyzing systems and methods using acoustic radiation pressure
Michael D Ward, Eugene, OR (US); and Gregory Kaduchak, Chandler, AZ (US)
Assigned to Life Technologies Corporation, Carlsbad, CA (US)
Filed by Life Technologies Corporation, Carlsbad, CA (US)
Filed on Feb. 26, 2021, as Appl. No. 17/186,296.
Application 15/239,948 is a division of application No. 14/464,763, filed on Aug. 21, 2014, granted, now 9,476,855, issued on Oct. 25, 2016.
Application 14/464,763 is a division of application No. 12/239,453, filed on Sep. 26, 2008, granted, now 8,846,408, issued on Sep. 30, 2014.
Application 17/186,296 is a continuation of application No. 16/282,793, filed on Feb. 22, 2019, granted, now 10,969,325.
Application 16/282,793 is a continuation of application No. 15/239,948, filed on Aug. 18, 2016, granted, now 10,254,212, issued on Apr. 9, 2019.
Application 12/239,453 is a continuation in part of application No. PCT/US2008/059181, filed on Apr. 2, 2008.
Claims priority of provisional application 61/026,082, filed on Feb. 4, 2008.
Claims priority of provisional application 60/909,704, filed on Apr. 2, 2007.
Prior Publication US 2021/0364407 A1, Nov. 25, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 15/1404 (2024.01); A61M 1/36 (2006.01); G01N 15/12 (2006.01); G01N 21/64 (2006.01); G01N 27/447 (2006.01); G01N 29/22 (2006.01); G01N 33/569 (2006.01); G01N 15/14 (2006.01); G01N 15/1409 (2024.01); G01N 21/17 (2006.01)
CPC G01N 15/1404 (2013.01) [A61M 1/3678 (2014.02); G01N 15/12 (2013.01); G01N 21/64 (2013.01); G01N 21/645 (2013.01); G01N 27/447 (2013.01); G01N 29/222 (2013.01); G01N 33/56966 (2013.01); G01N 15/1409 (2024.01); G01N 2015/1415 (2013.01); G01N 2015/142 (2013.01); G01N 15/147 (2013.01); G01N 21/1702 (2013.01); G01N 21/6428 (2013.01); G01N 2291/02416 (2013.01); G01N 2291/02809 (2013.01); G01N 2291/02836 (2013.01); Y10T 436/101666 (2015.01); Y10T 436/25 (2015.01); Y10T 436/25375 (2015.01); Y10T 436/25625 (2015.01); Y10T 436/2575 (2015.01)] 16 Claims
OG exemplary drawing
 
1. A method, comprising:
introducing a fluid comprising a population of particles therein to a flow path defining an axis therein;
applying an acoustic gradient at a gradient zone within the flow path to the population of particles so as to manipulate affected particles relative to the axis of the flow path;
interrogating at least some of the affected particles at an interrogation site with an interrogation source so as to obtain an optical signal from the interrogated particles; and
imaging interrogated particles having optical signals meeting user-defined criteria.