US 12,188,852 B2
Laser capture microdissection visualization chemistry
Lance A. Liotta, Bethesda, MD (US); Alessandra Luchini Kunkel, Burke, VA (US); Marissa Ashton Howard, Richmond, VA (US); Amanda Haymond Still, Manassas, VA (US); Amanda Lynn Still, Herndon, VA (US); and Virginia Espina, Fairfax, VA (US)
Assigned to George Mason Research Foundation, Inc.
Appl. No. 18/036,283
Filed by George Mason Research Foundation, Inc., Fairfax, VA (US)
PCT Filed Nov. 10, 2021, PCT No. PCT/US2021/058788
§ 371(c)(1), (2) Date May 10, 2023,
PCT Pub. No. WO2022/103843, PCT Pub. Date May 19, 2022.
Claims priority of provisional application 63/112,321, filed on Nov. 11, 2020.
Prior Publication US 2023/0384187 A1, Nov. 30, 2023
Int. Cl. G01N 1/30 (2006.01); G01N 1/28 (2006.01); G02B 21/33 (2006.01)
CPC G01N 1/30 (2013.01) [G02B 21/33 (2013.01); G01N 2001/284 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A liquid, comprising: a) an organic component; b) a stabilization component; and c) a preservation component, wherein the organic component, the stabilization component, and the preservation component are different components;
wherein the organic component is an anhydrous solution comprising less than 1% water content and comprises a volatile organic solution comprising acetonitrile, wherein the volatile organic solution has a refractive index of 1.5 or below;
wherein the volatile organic solution is not isopropanol, toluene, hexane, tetrahydrofuran and xylene;
wherein the stabilization component comprises a hygroscopic salt;
wherein the preservation component comprises a denaturant chemical component comprising a chaotropic agent; and
wherein the liquid is a visualization solution that is configured to improve resolving power of a lens by 1/1.5.