	US 12,188,099 B2
	Integrated proviral sequencing assay
Kevin Joseph, Pittsburgh, PA (US); and John W. Mellors, Pittsburgh, PA (US)
Assigned to UNIVERSITY OF PITTSBURGH-OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION, Pittsburgh, PA (US)
Appl. No. 17/413,130
Filed by UNIVERSITY OF PITTSBURGH-OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION, Pittsburgh, PA (US)
PCT Filed Dec. 12, 2019, PCT No. PCT/US2019/065945 § 371(c)(1), (2) Date Jun. 11, 2021, PCT Pub. No. WO2020/123787, PCT Pub. Date Jun. 18, 2020.
Claims priority of provisional application 62/778,480, filed on Dec. 12, 2018.
Prior Publication US 2022/0042120 A1, Feb. 10, 2022
Int. Cl. C12Q 1/70 (2006.01); C12Q 1/686 (2018.01)

CPC C12Q 1/703 (2013.01) [C12Q 1/686 (2013.01)]

15 Claims

1. A synthetic 71 base pair, partially-double stranded DNA oligonucleotide comprising a 3′dT overhang as set forth in SEQ ID NO: 1 and/or SEQ ID NO: 39, and 14 base pair antisense strand with a 5′P and two terminal 3′ C3 spacers on the antisense strand as set forth in SEQ ID NO: 2 and/or SEQ ID NO: 40.