US 12,188,090 B2
Method for discriminating sex of yellowtail group
Takashi Sakamoto, Tokyo (JP); Masatoshi Nakamoto, Tokyo (JP); Kiyoshi Kikuchi, Tokyo (JP); and Takashi Koyama, Tokyo (JP)
Assigned to National University Corporation Tokyo University of Marine Science and Technology, Tokyo (JP)
Appl. No. 16/970,697
Filed by National University Corporation Tokyo University of Marine Science and Technology, Tokyo (JP)
PCT Filed Feb. 18, 2019, PCT No. PCT/JP2019/005821
§ 371(c)(1), (2) Date Oct. 26, 2020,
PCT Pub. No. WO2019/160136, PCT Pub. Date Aug. 22, 2019.
Claims priority of application No. 2018-027260 (JP), filed on Feb. 19, 2018.
Prior Publication US 2021/0032697 A1, Feb. 4, 2021
Int. Cl. C12Q 1/6879 (2018.01)
CPC C12Q 1/6879 (2013.01) [C12Q 2600/124 (2013.01); C12Q 2600/156 (2013.01)] 3 Claims
 
1. A method for identifying sex of at least one yellowtail, comprising the steps of:
identifying whether the 169th nucleotide residue in exon 3 of HSD17B1 gene of the at least one yellowtail is an adenine (A) residue or a guanine (G) residue,
determining the sex of the at least one yellowtail based on the result of the identifying step, and
separating the at least one yellowtail into male or female based on the result of the determining step and culturing the male and female yellowtails separately,
wherein the at least one yellowtail is at least one selected from the group consisting of Japanese amberjack, Greater amberjack and Yellowtail amberjack,
wherein the exon 3 of the HSD17B1 gene of Japanese amberjack is set forth in SEQ ID NO: 3,
wherein the exon 3 of the HSD17B1 gene of Greater amberjack is set forth in SEQ ID NO: 6,
wherein the exon 3 of the HSD17B1 gene of Yellowtail amberjack is set forth in SEQ ID NO: 9,
wherein the sex of the yellowtail is determined to be male when the genotype at the 169th nucleotide residue in the exon 3 of the HSD17B1 gene is an A/A, and
wherein the sex of yellowtail is determined to be female when the genotype at the 169th nucleotide residue in the exon 3 of the HSD17B1 gene is a G/G or an A/G.