US 12,188,089 B2
Cleavable disulfide linkers and uses thereof
Ronald Graham, Carlsbad, CA (US); and Megha Cila, San Diego, CA (US)
Assigned to Singular Genomics Systems, Inc., San Diego, CA (US)
Filed by Singular Genomics Systems, Inc., San Diego, CA (US)
Filed on Feb. 12, 2024, as Appl. No. 18/439,062.
Application 18/439,062 is a continuation of application No. 18/449,601, filed on Aug. 14, 2023, granted, now 11,946,103.
Application 18/449,601 is a continuation of application No. PCT/US2022/052357, filed on Dec. 9, 2022.
Claims priority of provisional application 63/311,607, filed on Feb. 18, 2022.
Claims priority of provisional application 63/301,775, filed on Jan. 21, 2022.
Claims priority of provisional application 63/288,369, filed on Dec. 10, 2021.
Prior Publication US 2024/0327907 A1, Oct. 3, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/68 (2018.01); C07H 19/10 (2006.01); C07H 19/20 (2006.01); C12Q 1/6874 (2018.01); C12Q 1/6876 (2018.01); C12Q 1/6806 (2018.01)
CPC C12Q 1/6874 (2013.01) [C07H 19/10 (2013.01); C07H 19/20 (2013.01); C12Q 1/6876 (2013.01); C12Q 1/6806 (2013.01)] 20 Claims
 
1. A method of incorporating a compound into a primer, the method comprising:
(i) contacting a primer with a compound, wherein said primer is hybridized to a nucleic acid molecule in a cell or tissue, wherein the compound comprises a detectable label attached thereto via a divalent linker, L100, wherein L100 comprises the formula:

OG Complex Work Unit Chemistry
wherein R5 and R6 are independently unsubstituted alkyl, —CN, —OH, —NH2, —COOH, —CONH2, —NO2, —SO3H, —SO4H, —SO2NH2, —NHNH2, —ONH2, substituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl, or substituted or unsubstituted heteroaryl; and W1 and W2 are —O—; and
(ii) incorporating with a polymerase the compound into the primer.