US 12,188,078 B2
Methods for preparing a sample for nucleic acid amplification using tagmentation
Louise Fraser, Cambridge (GB); Paula Kokko-Gonzales, Cambridge (GB); and Andrew Slatter, Cambridge (GB)
Assigned to Illumina Cambridge Limited, Cambridge (GB)
Filed by Illumina Cambridge Limited, Nr Saffron Walden (GB)
Filed on Sep. 15, 2021, as Appl. No. 17/447,794.
Application 17/447,794 is a continuation of application No. 17/005,128, filed on Aug. 27, 2020, granted, now 11,142,786.
Application 17/005,128 is a continuation of application No. 16/163,005, filed on Oct. 17, 2018, granted, now 10,774,367, issued on Sep. 15, 2020.
Application 16/163,005 is a continuation of application No. 14/733,452, filed on Jun. 8, 2015, abandoned.
Claims priority of provisional application 62/171,814, filed on Jun. 5, 2015.
Claims priority of provisional application 62/171,908, filed on Jun. 5, 2015.
Claims priority of application No. 1410196 (GB), filed on Jun. 9, 2014; and application No. 1412207 (GB), filed on Jul. 9, 2014.
Prior Publication US 2022/0002783 A1, Jan. 6, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 19/34 (2006.01); C12N 15/10 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6844 (2018.01)
CPC C12Q 1/6806 (2013.01) [C12N 15/1003 (2013.01); C12P 19/34 (2013.01); C12Q 1/6846 (2013.01)] 19 Claims
 
1. A method of sequencing nucleic acids from a cellular sample, comprising:
lysing cells from a nucleic acid-containing cellular sample with a lysis reagent to form a lysate of nucleic acids;
amplifying the nucleic acids in the lysate wherein there is no purification of the nucleic acids from the lysate prior to the amplification, wherein amplifying comprises tagmentation; and
sequencing the nucleic acids,
wherein the sample is a blood sample or a formalin-fixed paraffin-embedded (FFPE) sample.