US 12,188,076 B2
Selective medium composition for detection of P. carotovorum and detection method using the same
Se-Wook Oh, Seoul (KR); Unji Kim, Seoul (KR); Ji Hye Baek, Namyangju-si (KR); and So-Young Lee, Yangju-si (KR)
Assigned to KOOKMIN UNIVERSITY INDUSTRY ACADEMY COOPERATION FOUNDATION, Seoul (KR)
Filed by KOOKMIN UNIVERSITY INDUSTRY ACADEMY COOPERATION FOUNDATION, Seoul (KR)
Filed on Oct. 19, 2022, as Appl. No. 17/968,831.
Claims priority of application No. 10-2022-0006269 (KR), filed on Jan. 17, 2022; and application No. 10-2022-0038487 (KR), filed on Mar. 29, 2022.
Prior Publication US 2023/0227886 A1, Jul. 20, 2023
Int. Cl. C12Q 1/04 (2006.01); C12N 1/20 (2006.01); C12Q 1/10 (2006.01)
CPC C12Q 1/10 (2013.01) [C12N 1/20 (2013.01); C12N 2500/05 (2013.01); C12N 2500/34 (2013.01)] 8 Claims
OG exemplary drawing
 
1. A selective detection and isolation method for Pectobacterium carotovorum (P. carotovorum), the method comprising:
i) inoculating P. carotovorum strain into a medium composition consisting of water, pectin, cellobiose, inositol, bile salt, bromocresol green, agar, yeast extract, and sodium chloride (NaCl) for selective detection of the P. carotovorum and then culturing the strain;
ii) checking a pattern or circles of color development of colonies formed in the selective medium composition after inoculation or culture in step i); and
iii) selectively isolating the colonies having a light yellow-colored center with a cyan-colored periphery surrounding the light yellow-colored center which indicate the P. carotovorum only.