US 12,188,073 B2
Yeast cells and methods for producing fatty alcohols
Leopold D'Espaux, San Francisco, CA (US); and Jay D. Keasling, Berkeley, CA (US)
Assigned to THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, Oakland, CA (US)
Filed by The Regents of the University of California, Oakland, CA (US)
Filed on Jun. 21, 2022, as Appl. No. 17/845,842.
Application 17/845,842 is a continuation of application No. 16/786,558, filed on Feb. 10, 2020, granted, now 11,365,431.
Application 16/786,558 is a continuation of application No. 15/979,144, filed on May 14, 2018, granted, now 10,557,152, issued on Feb. 11, 2020.
Claims priority of provisional application 62/505,725, filed on May 12, 2017.
Prior Publication US 2023/0039548 A1, Feb. 9, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 7/04 (2006.01); C12N 9/00 (2006.01); C12N 9/02 (2006.01); C12N 9/10 (2006.01); C12N 15/52 (2006.01)
CPC C12P 7/04 (2013.01) [C12N 9/0008 (2013.01); C12N 9/1029 (2013.01); C12N 9/93 (2013.01); C12N 15/52 (2013.01); C12Y 101/01001 (2013.01); C12Y 102/01084 (2015.07); C12Y 203/0102 (2013.01); C12Y 203/01085 (2013.01); C12Y 604/01002 (2013.01)] 17 Claims
 
1. A genetically modified yeast cell comprising the following modifications: (a) a first nucleic acid encoding a Mus musculus fatty acid reductase (FAR)(MmFAR1) or Tyto alba FAR1 (TaFAR1) operatively linked to a promoter, and (b) a second nucleic acid encoding acetyl-CoA carboxylase (ACC1) operatively linked to a promoter and (c) (i) a reduced expression of or knocked out for a homolog of fatty acid aldehyde (HFD1), (ii) a reduced expression of or knocked out for an alcohol dehydrogenase (ADH6), (iii) a nucleic acid encoding a mutant of a bottleneck enzyme encoded by ACC1 insensitive to post-transcriptional and post-translational repression (ACC1**) operatively linked to a promoter, (iv) a reduced expression of or knocked out for a glutamate dehydrogenase (GDH1), or (v) a nucleic acid encoding a fatty acid-desaturase (OLE1) operatively linked to a promoter; wherein the MmFAR1 or TaFAR1, and ACC1 have an increased expression compared to expression of each corresponding MmFAR1 or TaFAR1, and ACC1 in an unmodified yeast cell, and the genetically modified yeast cell, when grown in 1% yeast extract, 2% peptone, and 2% dextrose (YPD) medium, is capable of producing at least 1.0 g of fatty alcohol per liter of medium.