US 12,188,070 B2
Method for preparing fermentable sugars from lignocellulosic biomass
Martin Dan Jeppesen, Odense (DK); Jan Larsen, Tommerup (DK); Kit Haubjerg Mogensen, Fredericia (DK); Anna Frederike Gossmann, Flensburg (DK); Anna Granly Hansen, Fredericia (DK); Laila Thirup, Skanderborg (DK); Lars Villadsgaard Toft, Silkeborg (DK); and Hanne Risbjerg Sørensen, Holte (DK)
Assigned to New Energy Blue LLC, Lancaster, PA (US)
Appl. No. 16/347,091
Filed by Inbicon A/S, Fredericia (DK)
PCT Filed Nov. 6, 2017, PCT No. PCT/EP2017/078340
§ 371(c)(1), (2) Date May 2, 2019,
PCT Pub. No. WO2018/083301, PCT Pub. Date May 11, 2018.
Claims priority of provisional application 62/417,570, filed on Nov. 4, 2016.
Prior Publication US 2019/0271017 A1, Sep. 5, 2019
Int. Cl. C12P 19/02 (2006.01); C07G 1/00 (2011.01); C08L 95/00 (2006.01); C12P 7/10 (2006.01); C12P 19/14 (2006.01); C13K 1/02 (2006.01); C13K 13/00 (2006.01)
CPC C12P 19/02 (2013.01) [C07G 1/00 (2013.01); C08L 95/00 (2013.01); C12P 7/10 (2013.01); C12P 19/14 (2013.01); C13K 1/02 (2013.01); C13K 13/002 (2013.01); C12P 2201/00 (2013.01); C12P 2203/00 (2013.01); Y02E 50/10 (2013.01)] 18 Claims
 
1. A method for providing a C5/C6 product from a lignocellulosic material, comprising the steps of:
a. Autohydrolysis pretreatment of the lignocellulosic material;
b Solid/liquid separation of the pretreated lignocellulosic material from step (a) into a first solid fraction and a first liquid fraction;
c. Enzymatic fiber hydrolysis of said first solid fraction from step (b) by use of an enzyme composition capable of degrading lignocellulosic material, thereby providing a C5/C6 fiber slurry comprising C5 and/or C6 sugars;
d. Solid/liquid separation of at least a portion of the C5/C6 fiber slurry from step (c) into a second solid fraction and a second liquid fraction; and
e. Combining at least a portion of said first liquid fraction and at least a portion of said second liquid fraction for enzymatic mixed sugar hydrolysis (MSH), whereby a MSH C5/C6 product is provided,
wherein xylose yield of the MSH C5/C6 product from step (e) is at least 60% of theoretical xylose yield.