	US 12,188,067 B2
	Method for biosynthesising diosmetin and/or hesperetin in a microorganism
Cyrille Pauthenier, Juvisy-sur-Orge (FR); André Le Jeune, Draveil (FR); Hélène Scornec, Longjumeau (FR); Célia Roussel, Saintry sur Seine (FR); and Laetitia Joubert, Palaiseau (FR)
Assigned to ABOLIS BIOTECHNOLOGIES, Evry (FR)
Appl. No. 17/429,463
Filed by ABOLIS BIOTECHNOLOGIES, Evry (FR)
PCT Filed Feb. 11, 2020, PCT No. PCT/EP2020/053493 § 371(c)(1), (2) Date Aug. 9, 2021, PCT Pub. No. WO2020/165182, PCT Pub. Date Aug. 20, 2020.
Claims priority of application No. 19305162 (EP), filed on Feb. 11, 2019.
Prior Publication US 2021/0355517 A1, Nov. 18, 2021
Int. Cl. C12P 17/06 (2006.01); C12N 1/18 (2006.01); C12N 9/10 (2006.01); C12R 1/865 (2006.01)

CPC C12P 17/06 (2013.01) [C12N 1/185 (2021.05); C12N 9/1007 (2013.01); C12R 2001/865 (2021.05); C12Y 201/01042 (2013.01)]

19 Claims

1. A recombinant microorganism comprising a heterologous nucleic acid sequence coding for an O-methyltransferase (OMT) which methylates eriodictyol and/or luteolin in position 4′, wherein the O-methyltransferase (OMT) is selected from an enzyme comprising a sequence selected from the group consisting of SEQ ID NOs: 87, 89, 91 and 93 or comprising a sequence having at least 90% identity with a sequence selected from the group consisting of SEQ ID NOs: 87, 89, 91 and 93 and having O-methyltransferase activity, and wherein the microorganism is a yeast.