US 12,188,057 B2
Primer-independent DNA polymerases and their use for DNA synthesis
Margarita Salas Falgueras, Madrid (ES); Modesto Redrejo Rodriguez, Madrid (ES); Mart Krupovic, Paris (FR); and Patrick Forterre, Paris (FR)
Assigned to CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS (CSIC), Madrid (ES); and INSTITUT PASTEUR, Paris (FR)
Appl. No. 16/756,812
Filed by CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS (CSIC), Madrid (ES); and INSTITUT PASTEUR, Paris (FR)
PCT Filed Oct. 19, 2018, PCT No. PCT/EP2018/078737
§ 371(c)(1), (2) Date Apr. 16, 2020,
PCT Pub. No. WO2019/077119, PCT Pub. Date Apr. 25, 2019.
Claims priority of application No. ES201731236 (ES), filed on Oct. 20, 2017.
Prior Publication US 2020/0263151 A1, Aug. 20, 2020
Int. Cl. C12N 9/12 (2006.01); C12Q 1/6844 (2018.01)
CPC C12N 9/1252 (2013.01) [C12Q 1/6844 (2013.01); C12Y 207/07007 (2013.01)] 11 Claims
 
1. A recombinant DNA polymerase enzyme comprising an amino acid sequence of a parental DNA polymerase of family B wherein the KxY motif has been substituted by a peptide consisting of the amino acid sequence SEQ ID NO: 1, wherein the recombinant DNA polymerase enzyme has DNA polymerase activity and primase activity, wherein the parental DNA polymerase of family B is a Bam35 DNA polymerase or a phi-29 DNA polymerase, and wherein the recombinant DNA polymerase enzyme comprises the amino acid sequence SEQ ID NO: 3 or SEQ ID NO: 4.