	US 12,188,038 B2
	Methods, devices and systems for enhanced transduction efficiency
Reginald Tran, Atlanta, GA (US); Wilbur Lam, Atlanta, GA (US); David Myers, Atlanta, GA (US); Christopher Doering, Atlanta, GA (US); and Harold Spencer, Marietta, GA (US)
Assigned to Emory University, Atlanta, GA (US); Children's Healthcare of Atlanta, Inc., Atlanta, GA (US); and Georgia Tech Research Corporation, Atlanta, GA (US)
Filed by Emory University, Atlanta, GA (US); Georgia Tech Research Corporation, Atlanta, GA (US); and Children's Healthcare of Atlanta, Inc., Atlanta, GA (US)
Filed on Nov. 26, 2019, as Appl. No. 16/696,456.
Application 16/696,456 is a continuation of application No. 15/369,598, filed on Dec. 5, 2016, abandoned.
Claims priority of provisional application 62/263,137, filed on Dec. 4, 2015.
Prior Publication US 2020/0095608 A1, Mar. 26, 2020
Int. Cl. C12N 15/86 (2006.01); C12N 15/87 (2006.01)

CPC C12N 15/86 (2013.01) [C12N 15/87 (2013.01)]

20 Claims

1. A method of cell transduction, comprising:

simultaneously introducing a load of target cells and viral vectors into a channel of a device;

incubating the device for a period of time to transduce the target cells;

flowing a flushing solution through the channel to cause transduced cells to move toward an inlet/outlet of the device; and

collecting the transduced cells from the inlet/outlet;

wherein the channel has a cross-section, the cross-section having a height and a width, the width of the channel being greater than the height; and

wherein the height is about 50-250 μm.