US 12,188,034 B2
Transgenic corn event MON95275 and methods for detection and uses thereof
Sarah L. Brown, Weldon Spring, MO (US); Stanislaw Flasinski, Ballwin, MO (US); Aihong Pan, St. Louis, MO (US); Jason W. Stelzer, Wildwood, MO (US); Heidi M. Windler, Fenton, MO (US); and Yong Yin, Creve Coeur, MO (US)
Assigned to Monsanto Technology LLC, St. Louis, MO (US)
Filed by Monsanto Technology LLC, St. Louis, MO (US)
Filed on Jun. 1, 2022, as Appl. No. 17/829,963.
Application 17/829,963 is a division of application No. 17/235,129, filed on Apr. 20, 2021, granted, now 11,578,339.
Claims priority of provisional application 63/014,771, filed on Apr. 24, 2020.
Prior Publication US 2022/0364109 A1, Nov. 17, 2022
Int. Cl. C12N 15/82 (2006.01); C12Q 1/6895 (2018.01)
CPC C12N 15/8286 (2013.01) [C12Q 1/6895 (2013.01); C12Q 2600/13 (2013.01); C12Q 2600/158 (2013.01)] 5 Claims
 
1. A pair of DNA molecules, comprising a first DNA molecule and a second DNA molecule different from the first DNA molecule, that function as DNA primers when used together in an amplification reaction with a sample containing corn event MON95275 template DNA to produce an amplicon diagnostic for the presence of said corn event MON95275 DNA in said sample,
wherein said first DNA molecule comprises at least 15 contiguous nucleotides of SEQ ID NO:9 or a complement thereof,
wherein said second DNA molecule comprises at least 15 contiguous nucleotides of SEQ ID NO:11, SEQ ID NO: 12, or a complement thereof,
wherein said amplicon comprises the nucleotide sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO: 7, and SEQ ID NO:8.