	US 12,188,032 B2
	Transcription factor-mediated promotion of direct somatic embryogenesis
Jeroen Hofenk, Antwerp (BE); and James Troch, Antwerp (BE)
Assigned to PERPETUUM CROPSCIENCE BV, Antwerp (BE)
Appl. No. 17/437,741
Filed by PERPETUUM CROPSCIENCE BVBA, Antwerp (BE)
PCT Filed Mar. 11, 2020, PCT No. PCT/EP2020/056457 § 371(c)(1), (2) Date Sep. 9, 2021, PCT Pub. No. WO2020/182857, PCT Pub. Date Sep. 17, 2020.
Claims priority of application No. 19162059 (EP), filed on Mar. 12, 2019.
Prior Publication US 2022/0144900 A1, May 12, 2022
Int. Cl. C12N 15/82 (2006.01); A01H 4/00 (2006.01); C07K 14/415 (2006.01)

CPC C12N 15/8262 (2013.01) [A01H 4/002 (2021.01); A01H 4/005 (2013.01); C07K 14/415 (2013.01); C07K 2319/033 (2013.01); C07K 2319/09 (2013.01)]

13 Claims

1. A recombinant protein for use in a liquid culture medium for photo-autotrophic micropropagation of Cannabis sativa L., the recombinant protein comprising:

an Arabinogalactan protein and/or a plant transcription factor associated with plant growth and development, and

a cell penetrating peptide sequence and a nuclear localization signal, wherein said nuclear localization signal is encoded by the peptide sequence SEQ ID NO 1,

wherein said cell penetrating peptide sequence comprising a sequence of 7 to 25 arginines forming a poly-arginine transduction domain,

wherein said plant transcription factor comprises AP2-like ethylene-responsive transcription factor BBM from Arabidopsis thaliana, or an analog to said AP2-like ethylene-responsive transcription factor BBM from Arabidopsis thaliana, wherein said analog is codon-optimized for a host species.